Contribution of the csgA and bcsA genes to Salmonella enterica serovar Pullorum biofilm formation and virulence |
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| Authors: | Muhanad El Hag Zheng Feng Yangyang Su Xiao Wang Afrah Yassin Sujuan Chen |
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| Affiliation: | 1. College of Veterinary Medicine, Yangzhou University, Yangzhou, People’s Republic of China;2. Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, People’ Republic of China;3. Jiangsu Research Centre of Engineering and Technology for Prevention and Control of Poultry Disease, Yangzhou, People’ Republic of China;4. Faculty of Public and Environmental Health, University of Khartoum, Khartoum, Sudan;5. Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, People’ Republic of China |
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| Abstract: | ![]()
Salmonella biofilm formation is important to environmental stress resistance and virulence. However, the roles of the csgA and bcsA genes, which affect curli protein and cellulose production, respectively, in Salmonella enterica serovar Pullorum, are unknown. Here we constructed deletions in the csgA and bcsA genes in S. enterica serovar Pullorum strain S6702 and evaluated several aspects of biofilm formation and virulence. ΔcsgA showed decreased production of curli fimbriae, while ΔbcsA had reduced cellulose production. Both mutants had a reduced ability to form biofilms. ΔcsgA was reduced in adhesion and invasion to HeLa cells and exhibited decreased intracellular proliferation in HD11 macrophages. ΔbcsA exhibited increased proliferation in HD11 cells and replicated better in chicken spleens, as compared to the wild-type strain. ΔcsgA virulence was attenuated in assays involving oral challenge of one-day-old chickens. |
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| Keywords: | Salmonella enterica serovar Pullorum biofilm csgA bcsA mutant pathogenicity |
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