| Screening and analysis of differentially expressed genes of dermal papillae cells with aggregative behavior |
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| 作者姓名： | 宋志强 郝飞 杨卫兵 王继文 邹锋 |
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| 作者单位： | DepartmentofDermatology,SouthwestHospital,ThirdMilitaryMedicalUniversity,Chongqing400038,China |
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| 基金项目： | SupportedbytheNationalNaturalScienceFoundationofChina(30 2 0 0 2 4 9) |
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| 摘 要： | Objective: To screen and clone differentially expressed genes of dermal papillae cells (DPC) with aggregative behavior, and to explore the molecular mechanism of their aggregation. Methods: Total RNAs were extracted from DPC with and without aggregative behavior and double strand cDNAs were synthesized by using SMART cDNA synthesis, respectively.The cDNA fragments of differentially expressed genes in DPCs with aggregative behavior were isolated by suppression subtractive hybridization. Positive clones were screened by PCR method and verified by cDNA dot blot, Northern blot and then analyzed through homologous retrieving. Results: A subtractive cDNA library of DPC with aggregative behavior has been successfully constructed. The result of screening and cloniog of the library showed that, DPC with aggregative behavior could expresse genes related to homologous aggregation, proliferation and cycle control, including known genes (capping protein,paladin, vascular endothelial growth factor), hematopoietic stem/progenitor cells (HSPC) related clone ( HSPC011 and HSPC016) and a new gene. Conclusion: The construction of subtracted library of DPC lays solid foundation for screening and cloning new and specific genes related to aggregative behavior of DPC. Several genes might be cooperatively involved in the homologous aggregation, proliferation and cycle control of DPC.Among these genes, capping protein and palladin might be closely related to the aggregative behavior of dermal papilla cells, and VEGF and HSPC related clone would be responsible for the status of higher proliferation of dermal papilla cells.
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| 关 键 词： | 乳头状突起细胞 基因表达 PCR VEGF 细胞增殖 毛发滤泡 |
Screening and analysis of differentially expressed genes of dermal papillae cells with aggregative behavior |
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| Song Zhi qiang,Hao Fei ,Yang Wei bing,Wang Ji wen,Zou Feng.Screening and analysis of differentially expressed genes of dermal papillae cells with aggregative behavior[J].Journal of Medical Colleges of PLA(China),2004,19(1):15-21. |
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| Authors: | Song Zhi qiang Hao Fei Yang Wei bing Wang Ji wen Zou Feng |
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| Institution: | Song Zhi qiang,Hao Fei **,Yang Wei bing,Wang Ji wen,Zou Feng Department of Dermatology,Southwest Hospital,Third Military Medical University,Chongqing 400038,China |
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| Abstract: | Objective: To screen and clone differentially expressed genes of dermal papillae cells (DPC) with aggregative behavior, and to explore the molecular mechanism of their aggregation. Methods: Total RNAs were extracted from DPC with and without aggregative behavior and double strand cDNAs were synthesized by using SMART cDNA synthesis, respectively. The cDNA fragments of differentially expressed genes in DPCs with aggregative behavior were isolated by suppression subtractive hybridization. Positive clones were screened by PCR method and verified by cDNA dot blot, Northern blot and then analyzed through homologous retrieving. Results: A subtractive cDNA library of DPC with aggregative behavior has been successfully constructed. The result of screening and cloning of the library showed that, DPC with aggregative behavior could expresse genes related to homologous aggregation, proliferation and cycle control, including known genes (capping protein, paladin, vascular endothelial growth factor), hematopoietic stem/progenitor cells (HSPC) related clone (HSPC011 and HSPC016) and a new gene. Conclusion: The construction of subtracted library of DPC lays solid foundation for screening and cloning new and specific genes related to aggregative behavior of DPC. Several genes might be cooperatively involved in the homologous aggregation, proliferation and cycle control of DPC. Among these genes, capping protein and palladin might be closely related to the aggregative behavior of dermal papilla cells, and VEGF and HSPC related clone would be responsible for the status of higher proliferation of dermal papilla cells. |
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| Keywords: | dermal papilla cells suppression subtractive hybridization library |
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