电针"人中"穴的抗过敏性休克作用及与脑内神经降压肽含量的关系

目的 观察电针“人中”穴的抗过敏性休克作用及与脑内神经降压肽含量的关系，探讨电针“人中”穴的抗过敏性休克作用机制。方法 选用76只成年健康豚鼠，制成过敏性休克模型，以平均动脉压（MAP）为指标，观察同强度（电压3V）不同频率（4～16、30～40、80～100Hz）电针“人中”穴对过敏性休克低血压反应的影响，并用放射免疫法测定各脑区内神经降压肽（neurotensin，NT）的含量。结果 ①不同频率分别电针“人中”穴的升压效应均显著高于各自的非穴位电针对照组（P〈0.05，P〈0.01）；不同频率分别电针“人中”穴的三组之间以及非穴位电针三组之间MAP的变化差异无统计学意义。②正常组与过敏性休克组下丘脑、延髓、腺垂体、神经垂体中NT含量的变化差异均无统计学意义，而过敏性休克加不同频率电针“人中”穴各组的下丘脑、延髓、腺垂体、神经垂体中NT的含量均显著高于单纯休克组（P〈0．05，P〈0.01）。过敏性休克加不同频率电针“人中”穴三组间各脑区内NT含量的变化差异均无统计学意义。结论 ①电针“人中”穴对过敏性休克时的低血压反应确有迅速而良好的改善作用。②NT可能参与电针“人中”穴的抗过敏性休克作用。

Relationship between the Effect of Anti-anaphylactic Shock of Electroacupuncture at "Renzhong" and Neurotensin Content of the Brain in Guinea Pigs

Objective To explore the therapeutic effect of electroacupuncture(EA) on allergic shock in guinea pigs.Methods Experiments were performed on 76 anaphylactic shock model guinea pigs that were randomly divided into 4-16 Hz,30-40 Hz and 80-100 Hz groups.The 3 groups were further divided into EA of "Renzhong"(DU 26) and non-acupoint subgroups.Mean arterial pressure(MAP) was determined via right carotid catheter."Renzhong"(DU 26) was stimulated with different frequency and with stimulation duration of 30 min.The contents of neurotensin(NT) in hypothalamus,medulla,anteropituitary and postpituitary tissues were measured by radioimmunoassay.Results(1) The electroacupuncture at "Renzhong" point could produce a significant and rapid effect of antianaphylactic shock in comparison with non-acupoint group(P<0.05,P<0.01).(2) After EA stimulation,NT contents were increased significantly in brain in comparison with anaphylactic shock model group(P<0.05,P<0.01).Conclusion EA possesses a good pressor action in allergic shock guinea pigs,which may be mediated by NT in brain.