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| 应用免疫细胞化学和原位杂交诊断单纯疱疹病毒性脑炎 | |
| 引用本文： | 肖波,向传喜,牛好敏,王华,谢光洁,梁静慧,邓采菽,李柯娃,章蓓. 应用免疫细胞化学和原位杂交诊断单纯疱疹病毒性脑炎[J]. 卒中与神经疾病, 1994, 0(4) |
| 作者姓名： | 肖波 向传喜 牛好敏 王华 谢光洁 梁静慧 邓采菽 李柯娃 章蓓 |
| 作者单位： | 湖南医科大学湘雅医院神经内科，湖南医科大学电镜室，河南濮阳市人民医院，湖南省妇幼保健院 |
| 摘 要： | 用免疫细胞化学和原位杂交方法对２０例散发性脑炎脑活检组织进行了研究。结果３例确诊为单纯疱疹病毒性脑炎，其中２例单纯疱疹病毒ＤＮＡ阳性，１例抗原阳性。病毒ＤＮＡ主要分布于神经元和胶质细胞核内，胞浆内罕见；病毒抗原在神经元胞浆内较胞核多，在胶质细胞和单核细胞内则相反。研究表明二种方法联合应用，可提高散发性病毒性脑炎的病因检出阳性率。 |
| 关 键 词： | 脑炎，人疱疹病毒，脑活检，免疫细胞化学，原位杂交 |
Immumocytochemistry and in Situ Hybridization for Diagnosis of Herpes Simplex Virus Encephalitis |
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| Xiao Bo, Xiang Changxi,Niou Haomin, et al. Immumocytochemistry and in Situ Hybridization for Diagnosis of Herpes Simplex Virus Encephalitis[J]. Stroke and Nervous Diseases, 1994, 0(4) | |
| Authors: | Xiao Bo Xiang Changxi Niou Haomin et al |
| Abstract: | Herpes simphex virus was investigated by immunocytochemistry(ICC)and in situ hybridization(ISH)in formalin-fired and paraffin-embedded brain tissues of 20 cases with sporadic virus encephelitis. HSV DNA was found in 2 cases by ISH technique and one case gave a postive response in ICC. HSV DNA was Iocated in the nucleus. Virus antigens were discovered more easily in the cytoplasm of neuron than in the nucleus, but reversely in gilal cells and monocytes. These results demonstrated that combined use of two techniques can enhance the possibility to detect the cause of sporadic virus encephalitis. |
| Keywords: | Encephalitis Human herpes virus Brain biopsy Immunocytochemistry In situ hybridization |
| 本文献已被 CNKI 等数据库收录！ | |