清金得生片抗肿瘤作用的实验研究

目的]观察中药复方清金得生片(清金片)的抗肿瘤作用。方法]复制C57小鼠Lewis肺癌模型,以替加氟片(FT-207)为阳性对照药(0.2g/kg),观察清金片高、中、低刑量(3.15、1.05、0.35 g/kg)组的抑瘤率;复制NIH小鼠艾氏腹水癌(EAC)模型,观察清金片各剂量组的生存时间和生命延长率;以 8 g/kg清金片灌胃SD大鼠,制备含药血清,采用体外培养与四甲基偶氯唑盐(MTT)法,观察体积分数为10％、20％、30％的清金片含药血清对人肺腺癌细胞株(LAC)生长的抑制作用;观察清金片各剂量组时肉瘤180(S180)荷瘤小鼠二甲苯致炎的耳肿胀度,以及H22腹水瘤荷瘤小鼠气管内酚红排出量的影响。结果]清金片高、中、低剂量组在抑制小鼠Lewis肺癌生长方面作用与FT-207相仿,延长EAC荷瘤小鼠的存活时间和生命延长率的作用优于FT-207,高剂量组在减少二甲苯所致小鼠耳肿胀方面作用与FT-207及阿斯匹林相似,而在促进荷瘤小鼠气管内酚红排出量方面的作用优于FT-207而与溴已新片相仿;清金片各浓度含药血清均能抑制LAC的生长,其作用与模型组比较均有显著性差异(P<0.05或P<0.01),且均呈现剂量效应关系。结论]清金得生片对原发性支气管肺癌及转移性肺癌有抑瘤、抗炎、祛痰作用。其作用总体与化疗药FT-207相似,但在延长荷瘤小鼠的存活时间方面优于FT-207

Experimental Studies on Antitumor Action of Qingjin Desheng Tablets

Objective] To investigate the anti-tumor effect of Qingjin Desheng tablets (QDT). Methods] C57 mice models of Lewis pulmonary carcinoma were established to observed the tumor-inhibitor)' rate of QDT in three doses (3.15, 1.05 and 0.35g/kg respectively); the anti-tumor effect of QDT was compared with that of tegafur (FT-207) tablets. NIH mice models of Ehrlich ascites carcinoma (EAC) were established to observe the effect of QDT on survival time and life-prolongation rate. SD rats were given QDT 8g/kg by gastric injection and then serum contained QDT was prepared. In-vitro inhibitory effect of serum contained QDT (at volume percentages of 10%, 20% and 30% respectively) on human lung adenocarcinoma (LA) cell line was observed by thiazolyl blue colorimetry (MTT) . Effects of QDT on xylene-induced auricular swelling in S180 transplanted mice, as well as effects on bronchial phenol red excretion in H22 transplanted mice, were assayed. Results] The effects of three-dose QDT in inhibiting the growth of mice Lewis pulmonary carcinoma were similar to those of FT-207 but better than those of FT-207 in prolonging the survival time and life-prolongation rate in EAC mice. High-dose QDT was superior to FT-207 in decreasing xylene-induced auricular swelling and in increasing the excretion of bronchial phenol red in tumor-transplanted mice; however, its effects were similar to those of bromhexine. The effect of serum contained QDT at different concentrations differed from that in model group in inhibiting the growth of LA cell line ( P < 0.05 or P < 0.01) , the effect being in a dose-effect manner. Conclusion] QDT has antitumor, anti-inflammation and expectorant action on primary bronchial carcinoma carcinoma and metastatic pulmonary carcinoma; its effect is similar to FT-207 but is better in prolonging the survival time of tumor-transplanted mice.