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| HPLC-电雾式检测器(CAD)检测法测定脂质体中磷脂含量 | |
| 引用本文： | 姜庆伟,杨瑞,梅兴国. HPLC-电雾式检测器(CAD)检测法测定脂质体中磷脂含量[J]. 中国药学杂志, 2007, 42(23): 1794-1796 |
| 作者姓名： | 姜庆伟 杨瑞 梅兴国 |
| 作者单位： | 军事医学科学院毒物药物研究所,北京,100850 |
| 摘 要： | 目的建立HPLC-电雾式检测器(Charged Aerosol Detection,CAD)检测脂质体磷脂含量及降解产物的方法。方法选用几种类脂材料制备成空白脂质体注射剂及冻干粉针剂,利用HPLC-CAD测定脂质体磷脂膜中各种类脂的含量,采用反相C8色谱柱,流动相为甲醇-水,梯度洗脱,流速1 mL·min-1。结果4种类脂材料的平均回收率分别为99.2%,98.7%,99.5%,99.1%;RSD分别为1.1%,1.5%,0.8%,1.3%。结论实验充分证明了HPLC-电雾式检测器联合应用,可以快速、高效、准确地检测脂质体中的磷脂含量及杂质。 |
| 关 键 词： | 脂质体 二棕榈酰磷脂酰胆碱 二硬脂酰磷脂酰甘油 单棕榈酰磷脂酰胆碱 二硬脂酰磷脂酰乙醇胺-聚乙二醇 高效液相色谱法 |
| 文章编号： | 1001-2494（2007）23-1794-03 |
| 收稿时间： | 2006-12-11 |
| 修稿时间： | 2006-12-11 |
Determination of Phospholipid and Its Degradation Products in Liposomes for Injection by HPLC- Charged Aerosol Detection (CAD) |
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| JIANG Qing-wei,YANG Rui,MEI Xing-guo. Determination of Phospholipid and Its Degradation Products in Liposomes for Injection by HPLC- Charged Aerosol Detection (CAD)[J]. Chinese Pharmaceutical Journal, 2007, 42(23): 1794-1796 | |
| Authors: | JIANG Qing-wei YANG Rui MEI Xing-guo |
| Affiliation: | Institute of Pharmacology and Toxicology，Academy of Military Medical Sciences，Beijing 100850，China |
| Abstract: | OBJECTIVE To develop a HPLC-CAD method for the determination of DSPE-PEG,phosphatidylcholine(DPPC),DSPG,lysophosphatidylcholine(MPPC) in phospholipid bilayer of blank liposomes for injection.METHODS A separation was performed on a C8 column with the mobile phase of methanol-water gradient elution at the flow rate of 1 mL·min-1. RESULTS The average recoveries of DSPE-PEG,DSPG,MPPC and DPPC were 99.2%,98.7%,99.5% and 99.1% and with RSDs of 1.1%,1.5%,0.8% and 1.3% respectively.CONCLUSION HPLC-CAD,a new method for phospholipids analysis,provides high sensitivity and reproducibility. |
| Keywords: | liposome DSPG MPPC DPPC DSPE-PEG HPLC |
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