清肝降压饮对自发性高血压大鼠心肌eNOS表达的影响

目的：观察清肝降压饮防治自发性高血压大鼠（SHR）心肌肥厚的作用,并探讨其作用机制。方法：将SHR随机分为模型组、清肝降压饮低剂量组、清肝降压饮高剂量组和西药组,并用Wistar大鼠作空白对照。灌胃4周后,利用免疫组化方法检测大鼠心肌内皮型一氧化氮合酶（eNOS）的表达。结果：各给药组大鼠血压、左室重量指数均较模型组显著降低（P〈0.01）,同时各给药组大鼠心肌细胞eNOS的表达均较模型组显著升高（P〈0.01）,其中清肝降压饮高剂量组效果最显著。结论：清肝降压饮可通过促进SHR心肌细胞eNOS的表达,降低SHR的血压和左心室重量指数,产生防治高血压心肌肥厚的效应。

Effects of clearing liver-heat and antihypertensive recipe on expression of eNOS in myocardium of spontaneously hypertensive rats

Objective：To observe the effects of clearing liver-heat and antihypertensive recipe（Chinese medicine） on myocardial hypertrophy of spontaneously hypertensive rats（SHRs） and explore the mechanism. Methods：SHRs were randomly divided into model group,low-dose group of Chinese medicine,high-dose group of Chinese medicine and western medicine group. Wistar rats were selected as control group. Following 4 weeks＇ intragastric administration,the expression of endothelial nitric oxide synthase（eNOS） in myocardium were detected by immuno-histochemistry technique. Results：The blood pressure and the left ventricular mass index（LVMI） of every treated group were decreased（P 0.01）,the expression of eNOS of every treated group was upregulated,and the high-dose group of Chinese medicine had the most significant effect. Conclusion：Clearing liver-heat and antihypertensive recipe could prevent and control myocardial hypertrophy of hypertension by increasing the expression of eNOS in myocardium and decreasing the blood pressure and LVMI.