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| 经前期综合征肝气逆证猕猴脑组织差减cDNA文库的构建 | |
| 引用本文： | 乔寒子,范立昌,魏盛,张惠云. 经前期综合征肝气逆证猕猴脑组织差减cDNA文库的构建[J]. 山东中医学院学报, 2010, 0(6): 526-529 |
| 作者姓名： | 乔寒子 范立昌 魏盛 张惠云 |
| 作者单位： | [1]广州中医药大学附属第二临床医学院,广东广州510120 [2]山东省莒县人民医院,山东莒县276500 [3]山东中医药大学中医药经典理论教育部重点实验室,山东济南250355 |
| 基金项目： | “973”计划资助课题（编号：2006CB504804）：山东省科技攻关项目课题（编号：2006GGB02001） |
| 摘 要： | 构建经前期综合征（PMS）肝气逆证猕猴与正常猕猴大脑边缘系统正向与反向差减文库,并对猕猴cDNA文库进行了系统测序和分析,以期筛选出与PMS肝气逆证相关的基因,尤其是与PMS肝气逆证有关的一些神经递质、性激素受体的基因。差减文库构建成功,为弄清PMS肝气逆证差异表达基因奠定了基础。 |
| 关 键 词： | PMS肝气逆证 猕猴模型 差减cDNA文库 抑制性消减杂交 |
Construction of the subtractive eDNA library of prcmenstrual syndrome macaque with liver-Qi invasion syndrome |
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| QIAO Han-zi,FAN Li-chang,WEI Sheng,ZHANG Hui-yun. Construction of the subtractive eDNA library of prcmenstrual syndrome macaque with liver-Qi invasion syndrome[J]. , 2010, 0(6): 526-529 | |
| Authors: | QIAO Han-zi FAN Li-chang WEI Sheng ZHANG Hui-yun |
| Affiliation: | 1.Second Affiliated Hospital,Guangzhou University of Traditional Chinese Medicine,Guangzhou 510120,China;2.Juxian People＇s Hospital,Juxian 276500,China;3. Key Laboratory of Traditional Chinese Medicine Classics Theory,Shandong University of Traditional Chinese Medicine, Jinan 250355, China) |
| Abstract: | We constructed limbic system subtractive cDNA library of normal macaque and premenstrual syndrome （PMS） macaque with liver-Qi invasion syndrome. The gene sequences in macaque cDNA library were detected and analyzed systematically. Some genes especially neurotransmitter and hormone receptors genes were selected which related to PMS with liver-Qi invasion syndrome. Subtractive cDNA library construction successfully provided solid foundation to clarify differential expression genes of PMS with liver-Qi invasion syndrome. |
| Keywords: | PMS liver-Qi invasion syndrome macaque model subtractive cDNA library suppresion subtraetive hybridization |
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