| 电针对脊髓损伤后细胞凋亡的影响 |
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| 引用本文： | 张志英,崔云华,严振国. 电针对脊髓损伤后细胞凋亡的影响[J]. 中国组织工程研究与临床康复, 2002, 6(6): 818-819 |
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| 作者姓名： | 张志英 崔云华 严振国 |
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| 作者单位： | 上海中医药大学,上海,200032 |
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| 摘 要： | 目的探讨电针对脊髓损伤后细胞凋亡的影响。方法230～280g雄性SD大鼠42只,随机分为模型组、电针治疗组、甲基强的松龙治疗组各12只及假手术组6只。采用改良的Allen's垂击法致大鼠脊髓(T10)中度损伤,损伤后即刻分别进行督脉电针或甲基强的松龙治疗,模型组不做处理,于术后6、24h处死动物,假手术组仅切除椎板,不损伤脊髓。采用TUNEL染色法观察细胞凋亡,并进行图像定量统计学分析。结果电针和甲基强的松龙均可显著减少TUNEL阳性细胞数。结论电针可抑制脊髓损伤后细胞凋亡,减轻脊髓继发性损伤,有利于神经功能的恢复。
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| 关 键 词： | 脊髓损伤 细胞凋亡 电针 |
| 文章编号： | 1671-5926(2002)06-0818-02 |
| 修稿时间： | 2002-02-01 |
The effect of electric acupuncture on apoptosis in spinal cord injury |
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| ZHANG Zhi ying,CUI Yun hua,YAN Zhen guo. The effect of electric acupuncture on apoptosis in spinal cord injury[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2002, 6(6): 818-819 |
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| Authors: | ZHANG Zhi ying CUI Yun hua YAN Zhen guo |
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| Affiliation: | ZHANG Zhi ying,CUI Yun hua,YAN Zhen guo.Shanghai University of Traditional Chinese Medicine and Pharmacy,Shanghai 200032,China |
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| Abstract: | Objective To study the effect of electric acupuncture on apoptosis in spinal cord injury. Methods The rats were immediately treated in electric acupuncture after spinal cord injury(T10) by Allen's weight drop (50 g cm force) technique and killed at 6h and 24h after operation. The apoptosis of spinal slice was examined by the method of the terminal deoxynucleotidal transferase mediated dUTP biotin nick end labeling(TUNEL) reaction, the TUNEL positive cells were quantitatively analyzed by computer image analysis system. Result The number of TUNEL positive cells reduced significantly in spinal cord injured rats treated by electric acupuncture. Conclusion Electric acupuncture may prevent the progress of the second spinal cord injury by inhibits the apoptotic neuronal death after spinal cord injury. |
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| Keywords: | electric acupuncture apoptosis spinal cord injury |
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