水蓑衣提取物对裸鼠肝癌移植瘤生长的影响

Effects of extraction from Hygrophila salicifolia on hepatocarcinoma cell xenografts in nude mice

AIM: To assess the anti-proliferative effect of the extraction from Hygrophila salicifolia on hepatocellular carcinoma (HCC) cells, and to provide the experimental evidence for the use of Hygrophila salicifolia extraction in the therapy of hepatocarcinoma. METHODS: Twenty-four female nude mice were subcu-taneously injected with human HCC cells. After 14 days, the mice were divided equally into control group, low dose Hygrophila salicifolia group, high dose Hygrophila salicifolia group and hydroxycamptothecin group. The volume and weight of tumor were measured 20 days after treatment. Histological changes of the xenografts and the serum levels of AST, ALT, LDH, and CRE were determined. RESULTS: In comparison with the control group, the tumor volume and weight of both Hygrophila salicifolia groups were decreased significantly (P<0.05). The tumor inhibiton rates were 46% and 62% (P<0.05) in low- and high- dose group, respectively. Histological examination results from Hygrophila salicifolia groups were almost the same as those from hydroxycamptothecin group. There were no obvious difference in the weights and the serum levels of AST, ALT, LDH, CRE the mice treated with Hygrophila salicifolia and those treated with hydroxycamptothecin. CONCLUSION: The extraction from Hygrophila salicifolia can significantly inhibit the growth of hepatocarcinoma cell xenografts in nude mice in a dose-dependent manner. Its effects on the general condition and on the functions of the heart, the liver and the kidney are almost the same as those of hydroxycamptothecin.