Significance of combined detection of LunX mRNA and tumor markers in diagnosis of lung carcinoma<b class="markJ">简</b> Significance of combined detection of LunX mRNA and tumor markers in diagnosis of lung carcinoma期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Significance of combined detection of LunX mRNA and tumor markers in diagnosis of lung carcinoma简

引用本文：	Hui Yu,Xiuying Huang,Zhihua Zhu,Yi Hu,Wei Ou,Lanjun Zhang,Ningning Zhou. Significance of combined detection of LunX mRNA and tumor markers in diagnosis of lung carcinoma简[J]. 中国癌症研究, 2014, 26(1): 89-94. DOI: 10.3978/j.issn.1000-9604.2014.02.04

作者姓名：	Hui Yu Xiuying Huang Zhihua Zhu Yi Hu Wei Ou Lanjun Zhang Ningning Zhou

基金项目：	This study was supported by the Guangdong Medical Science and Technology Research Fund （A2009217）,the Fundamental Research Funds for the Central Universities and the grant from Youth Training Plan of Sun Yat-Sen University （No.10ykpy38）,the Research Award Fund for Outstanding Young Researchers in Sun Yat-Sen Cancer Center （Nos.3030451720 06 and 3030 45172005） and the Science ＆ Technology Pillar Program of Guangdong Province （No.2011B031800220,2012B031800371）.

摘要：	Objective：To evaluate the significance of combined detection of LunX mRNA,carcinoembryonic antigen （CEA）,neuron-specific enolase （NSE）,and cytokeratin 21-1 fragment （CYFRA21-1） in clinical diagnosis of lung carcinoma.Methods：Based on the quantitative RT-PCR and chemiluminescence immunoassay,the expression levels of LunX mRNA,CEA,NSE,and CYFRA21-1 in 113 patients with lung carcinoma （case group） and 30 healthy participants （control group） were detected.Meantime,the sensitivity,specificity,and accuracy of the combination detection were also explored.Results：The positive rates of LunX mRNA in peripheral blood and CEA,NSE,and CYFRA21-1 in serum were significandy higher in case group than those in control group （x2=17.295,16.825,19.148,and 17.450; P＜0.05）.There was no statistical significance when positive rate of LunX mRNA was evaluated among different pathological types （x2=0.047,P＞0.05）.The positive rate of LunX mRNA in stage Ⅰ ＋ Ⅱ,Ⅲ,and Ⅳ had a significandy increasing tendency （x2=10.565,32.462,P＜0.05）.The positive rate of CYFRA21-1 was highest in squamous carcinoma （78.5％）,the positive rate of NSE was highest in small cell carcinoma （86.7％）,and the positive rate of CEA wag highest in lung adenocarcinoma （80.4％）.The sensitivity and accuracy of the combination detection were 91.1％ and 88.1％,respectively.Conclusions：The combined detection of LunX mRNA and tumor markers （TMs） including CEA,NSE,and CYFRA21-1 in peripheral blood is helpful to increase the diagnostic accuracy of lmg cancer.Also,it can inform the pathological typing of lung carcinoma.
关键词：	mRNA表达联合检测肿瘤标志物临床诊断肺癌神经元特异性烯醇化酶化学发光免疫分析定量RT-PCR
收稿时间：	2013-12-02
Significance of combined detection of LunX mRNA and tumor markers in diagnosis of lung carcinoma

Hui Yu,Xiuying Huang,Zhihua Zhu,Yi Hu,Wei Ou,Lanjun Zhang,Ningning Zhou. Significance of combined detection of LunX mRNA and tumor markers in diagnosis of lung carcinoma[J]. Chinese Journal of Cancer Research, 2014, 26(1): 89-94. DOI: 10.3978/j.issn.1000-9604.2014.02.04

Authors:	Hui Yu Xiuying Huang Zhihua Zhu Yi Hu Wei Ou Lanjun Zhang Ningning Zhou

Affiliation:	[1]Department of Thoracic Surgery, Sun Yat-Sen University Cancer Center; State Key Laboratory of Oncology in South China; Collaborative Innovation Center for Cancer Medicine, Guangzhou 510060, P.R. China [2]Department of Medical Oncology, Sun Yat-Sen University Cancer Center; State Key Laboratory of Oncology in South China; Collaborative Innovation Center for Cancer Medicine, Guangzhou 510060, ER. China.

Abstract:	Objective To evaluate the significance of combined detection of LunX mRNA, carcinoembryonic antigen (CEA), neuron-specific enolase (NSE), and cytokeratin 21-1 fragment (CYFRA21-1) in clinical diagnosis of lung carcinoma. Methods Based on the quantitative RT-PCR and chemiluminescence immunoassay, the expression levels of LunX mRNA, CEA, NSE, and CYFRA21-1 in 113 patients with lung carcinoma (case group) and 30 healthy participants (control group) were detected. Meantime, the sensitivity, specificity, and accuracy of the combination detection were also explored. Results The positive rates of LunX mRNA in peripheral blood and CEA, NSE, and CYFRA21-1 in serum were significantly higher in case group than those in control group (^χ²=17.295, 16.825, 19.148, and 17.450; P<0.05). There was no statistical significance when positive rate of LunX mRNA was evaluated among different pathological types (^χ²=0.047, P>0.05). The positive rate of LunX mRNA in stage I + II, III, and IV had a significantly increasing tendency (^χ²=10.565, 32.462, P<0.05). The positive rate of CYFRA21-1 was highest in squamous carcinoma (78.5%), the positive rate of NSE was highest in small cell carcinoma (86.7%), and the positive rate of CEA wag highest in lung adenocarcinoma (80.4%). The sensitivity and accuracy of the combination detection were 91.1% and 88.1%, respectively. Conclusions The combined detection of LunX mRNA and tumor markers (TMs) including CEA, NSE, and CYFRA21-1 in peripheral blood is helpful to increase the diagnostic accuracy of lung cancer. Also, it can inform the pathological typing of lung carcinoma.

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Objective

Methods

Results

Conclusions