细胞凋亡与宫颈癌临床治疗效果关系的研究

目的：研究宫颈癌中细胞凋亡与放疗和放化疗临床效果的关系。方法：采用免疫组织化学法和脱氧核糖核酸转移酶介导的缺口末端标记（TUNEL）技术,检测60例宫颈癌患者治疗前后宫颈肿瘤细胞凋亡率。结果：在宫颈癌组织中,单纯放疗（radiotherapy,RT）前和放疗10 Gy后,肿瘤细胞凋亡率分别为33.33%和66.67%,有统计学差异（P〈0.05）;放化疗（synchronal radiochemotherapy,CRT）组治疗前和先行一次化疗再放疗10 Gy后,肿瘤细胞凋亡率分别为36.67%和93.33%,两者有统计学差异（P〈0.01）。治疗前组间对比阳性率无统计学差异（P〉0.05）,治疗后组间对比,放化疗组凋亡阳性率明显高于单纯放疗组,差异有统计学意义（P〈0.05）,且细胞凋亡与近期临床疗效呈正相关（r=0.823,P〈0.05）。结论：化疗对放射线诱导的宫颈癌细胞凋亡具有协同作用,细胞凋亡可作为宫颈癌放疗和放化疗疗效的预测指标,。

Study of the relationgship between apoptosis in cervical carcinoma and the clinical effect of treatment for patients

Objective:To study the realitionship between apoptosis and the clinical effect of treatment for patients with cervical carcinoma.Method:The immunohistochemical staining method and TdT mediated dUTP nick and labeling(TUNEL) technique were used to detect the changes of apoptosis in 60 cases of uteri cervix carcinoma during treatment.Results:Before and during the treatment,the positive rates of apoptosis increased,ranging from 33.33% to 66.67%(P<0.05) in the radiotherapy(RT) group and from 36.67% to 93.33%(P<0.05) in the chemoradiotherap(CRT) group respectively.There was a significant difference between the two groups.There was a significant correlation between the expression of apoptosis and the clinical effects(r=0.823,P<0.05,).Conclusion:Chemotherapy has cooperative effect on radition-induced apoptosis.Apoptosis may be a useful predictor of response to radiotherapy in carcinoma of the cervix.