5种中国罕见慢速生长非结核分枝杆菌标准株及临床分离株的药物敏感性分析

目的分别对熊本、慢生黄、施氏、苏尔加、三重分枝杆菌5种中国罕见分离的慢速生长非结核分枝杆菌标准株和临床分离株的药物敏感性进行分析。方法用微孔板Alamar blue显色法分别检测1株熊本分枝杆菌、3株慢生黄分枝杆菌、2株施氏分枝杆菌、2株苏尔加分枝杆菌、2株三重分枝杆菌等临床分离株及相应标准株对利福平、克拉霉素和阿米卡星等36种药物的最小抑菌浓度，判断分析其敏感性，并比较标准株及相应临床分离株对36种药物的敏感性差异。结果5种非结核分枝杆菌菌株中，多数对利福霉素类的利福喷丁和利福布丁、氨基糖苷类、多数氟喹诺酮类、卷曲霉素、大环内酯类（克拉霉素、罗红霉素和阿奇霉素）、利奈唑胺和氯法齐明等敏感。 三重分枝杆菌还对头孢菌素类药物表现出一定的敏感性。结论熊本、慢生黄、施氏、苏尔加、三重分枝杆菌等对克拉霉素等多种药物敏感，但有的临床分离株表现为耐药，治疗这些菌种感染时最好进行药物敏感性试验并联合用药。

Antibiotic susceptibility of reference and clinical strains of slowly growing nontuberculous mycobacteria of 5 species rarely isolated in China

ObjectiveTo explore the susceptibility of clinical isolates and reference strains of 5 species of nontuberculous mycobacteria, including Mycolicibacter kumamotonensis, Mycobacterium lentiflavum, Mycobacterium shimoidei, Mycobacterium szulgai, Mycobacterium triplex, which are rarely isolated in China.MethodsThe minimum inhibitory concentrations (MICs) of 36 antibiotics to 10 clinical isolates (1 strain of M. kumamotonensis, 3 strains of M. lentiflavum, 2 strains of M. shimoidei, 2 strains of M. szulgai and 2 strains of M. triplex) and 5 reference strains of each species were detected with microplate Alamar Blue assay. The drug susceptibility patterns of the strains were then analyzed, and the drug susceptibility patterns were compared between clinical isolates and reference strains.ResultsThe majority of the five species strains were sensitive to rifabutin, rifapentine, aminoglycosides, most of fluoroquinolones, capreomycin, clarithromycin, roxithromycin, azithromycin, linezolid and clofazimine. In addition, M. triplex strains were found to be sensitive to cephalosporins.ConclusionM. kumamotonensis, M. lentiflavum, M. shimoidei, M. szulgai and M. triplex were sensitive to multi antibiotics, while some clinical strains showed resistances. It is suggested that drug susceptibility test and combined drug therapy are necessary to treat the infections caused by these five species.