miR-451通过Akt负向调控糖尿病肾病患者组织上皮间质转分化

目的研究微小RNA-451(miR-451)对糖尿病肾病组织上皮间质转分化(EMT)的影响。方法取健康人肾脏组织和糖尿病患者肾脏组织,RT-qPCR检测肾组织mi-451、Akt和EMT相关基因(E-钙黏素、波形蛋白和α-平滑肌肌动蛋白)的表达;Western blot和免疫组织化学染色(IHC)检测Akt和EMT相关蛋白的表达。结果糖尿病肾病肾脏组织中miR-451和E-钙黏素mRNA表达量显著低于正常肾组织(P<0.05);Akt、vimentin和α-SMA mRNA表达量显著增多(P<0.05);Akt、vimentin和α-SMA蛋白表达量显著增高(P<0.05);E-钙黏素蛋白表达量显著降低(P<0.05)。结论糖尿病肾病时miR-451很可能通过Akt负向影响肾脏上皮间质转分化。

miR-451 negatively regulates EMT of the diabetic nephropathy by Akt

Objective To study microRNA-451(miR-451)'s influence on diabetic nephropathy epithelial mesenchymal transdifferentiation(EMT).Methods Taking human healthy kidney tissues and diabetic nephropathy kidney tissues,real-time PCR was used to detect the expression of miR-451,Akt and EMT related genes(E-cadhering,vimentin and alpha smooth muscle actin)in different groups;Western blot and immuno-histochemical staining(IHC)were used to detect the expression of Akt and EMT related proteins in different groups.Results The expressions of miR-451 and E-cadherin mRNA significantly reduced in diabetic nephropathy kidney tissues as compared with healthy kidney tissues,and the expressions of Akt,vimentin and alpha-SMA mRNA significantly increased.The expressions of Akt,vimentin and alpha-SMA protein significantly increased,and the expression of E-cadherin protein decreased significantly(P<0.05).Conclusions The miR-451 is likely to negatively influence diabetic nephropathy epithelial mesenchymal trans differentiation by Akt.