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| 脂质过氧化反应混合物诱发人羊膜FL系细胞程序外DNA合成 | |
| 引用本文： | 黄大鹏,关琏,苗健,陈本懋. 脂质过氧化反应混合物诱发人羊膜FL系细胞程序外DNA合成[J]. 郑州大学学报(医学版), 1989, 0(4) |
| 作者姓名： | 黄大鹏 关琏 苗健 陈本懋 |
| 作者单位： | 河南医科大学生物化学教研室(黄大鹏,关琏,苗健),河南医科大学生物化学教研室(陈本懋) |
| 摘 要： | 大鼠肝微粒体依赖NADP的脂质过氧化反应混合物(LPRM)可诱发人羊膜FL系细胞程序外DNA合成(UDS)。当一定浓度的LPRM(0.7～2.4mg微粒体蛋白/ml)加入~(14)C—TdR预标记的FL细胞培基时,引起羟基尿素(HU)抗性的程序外~3H—TdR掺入,与对照组相比差异显著(P<0.05),并存在剂量—效应关系。由此可见,脂质过氧化反应混合物中含有诱变物,推测其在癌肿发病中可能有直接作用。 |
| 关 键 词： | 大鼠肝微粒体 脂质过氧化 诱变性 程序外DNA合成 人羊膜FL细胞 |
The reaction mixture of lipid peroxidation inducing unscheduled DNA synthesis in human amnion FL cells |
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| Huang Dapeng et al. The reaction mixture of lipid peroxidation inducing unscheduled DNA synthesis in human amnion FL cells[J]. Journal of Zhengzhou University: Med Sci, 1989, 0(4) | |
| Authors: | Huang Dapeng et al |
| Affiliation: | Huang Dapeng et al Department of Biochemistry |
| Abstract: | The reaction mixture of NADPH-dependent lipid peroxidation of rat liver micro-somes induces"unscheduled DNA synthesis(UDS)" in human amnion FL cells. The re-sults obtained were as follows: (1)Lipid peroxidation of microsomes occurred extensi-vely in the incubation with NADPH and Fe~(2+). (2) In the FL cells, UDS level incres-ed markedly when the cells were incubated in the reaction microsomal lipid perox-idation.The induced UDS were dependent on the extent of the lipid peroxidation.According to the results, it is concluded that some DNA-damaging and mutagenic su-bstances are indeed produced in the degradation process of peroxidized polyunsaturat-ed fatty acids in liver microsomal lipids. |
| Keywords: | lipid peroxidation mutation unscheduled DNA synthesis human amnion FL cells |
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