白藜芦醇对大鼠肾脏缺血-再灌注损伤保护作用研究

目的：观察白藜芦醇(RES)对大鼠肾脏缺血-再灌注(I/ R)保护作用并初步探讨其相关机制。方法 SD 大鼠随机分为4组：空白对照组、I/ R 模型组、白藜芦醇(RES)处理组、RES + IR 模型组；检测各组大鼠血清肌酐(Cr)、尿素氮(BUN)及肾脏病理学改变,使用超氧化物歧化酶(SOD)活力、丙二醛(MDA)和谷胱甘肽过氧化物酶(GSH-PX)试剂盒检测肾脏组织 SOD、MDA、GSH-PX 产生；Western blot 法检测肾脏组织 p-AKT 蛋白水平。结果与空白对照组相比,I/ R模型组 Cr、BUN 含量均有升高,与 I/ R 模型组相比,RES + I/R 模型组 Cr、BUN 含量均有明显下降；病理学检测显示 RES+ I/ R 模型组肾脏损伤较 I/ R 模型组明显减轻,肾小球结构基本正常,细胞间质有少量炎性细胞浸润,基底膜稍有增厚；与空白对照组相比,I/ R 模型组 SOD、MDA、GSH-PX 含量均有升高,与 I/ R 模型组相比,RES + I/ R 模型组 SOD、GSH-PX含量均有明显下降； Western blot 检测显示,与空白对照组相比,I/ R 模型组 p-AKT 表达水平有所降低,但 RES 可提高 p-AKT 表达水平,差异有统计学意义。结论 RES 通过氧化应激保护 I/ R 肾脏损伤,其作用机制可能与 PI3K/ AKT 信号通路活化有关。

Resveratol protects on renal ischemical-reperfusion injury in rats

Objective To investigate the protective effects of resveratrol(RES) on renal ischemia-reperfusion(I/R) injury and explore its possible mechanisms in rats. Methods The SD rats were randomly divided into four groups: blank control group, I/ R model group, RES pretreatment group and RES + IR model group. We detected the contents of serum creatinine (Cr), blood urea nitrogen (BUN) and renal pathology in rat serum. Superoxide dismutase (SOD) activity stress, malondialdehyde (MDA) and glutathione peroxidase (GSH-PX) kit were used to detect renal SOD, MDA, GSH-PX contents. We detected the level of p-AKT protein in renal tissue by western blot. Results Compared to the blank control group, the contents of Cr and BUN were increased in I/ R model group. But compared to the I/ R model group, the contents of Cr and BUN were decreased significantly in RES + I/R model group. Pathology detection found that kidney damage reduced significantly in RES + I/ R model group com-pared with I/ R model group, such as glomerular structure presenting basically normal, stromal cells presenting a few infiltration and basement membrane slightly thickened. Compared to the blank control group, SOD, MDA, GSH-PX contents were increased in I/ R model group. But compared to I/ R model group, SOD, MDA, GSH-PX were decreased significantly in RES + I/ R model group. Compared to the blank control group, the expression of p-AKT was decreased in I/ R model group, and we also found that resveratrol could increase the level of AKT phos-phorylation, the difference was statistically significant. Conclusion Resveratrol protects I/ R renal injury via oxi-dative stress, and the mechanism may be related with the activation of PI3K/ AKT signal pathway.