羧基化聚乙烯基萘纳米微球的制备和应用

以乙烯基萘(VN)为聚合单体，采用乳液聚合法制备聚乙烯基萘(PVN)纳米微球。通过对其羧基化与β2微球蛋白(β2-M)抗体偶联，制成免疫检测试剂。分别用聚苯乙烯(PS)和 PVN 检测试剂测定β2-M 含量，数据经统计学处理和分析，对自制的 PVN 纳米微球胶乳增强免疫比浊( LE-TIA)检测试剂的性能进行评价。自制的羧基化 PVN 纳米微球免疫试剂成功对标准样本进行了检测并在一定范围内有较好的线性，比 PS 检测试剂更加灵敏。利用该研究采用的方法和条件可以成功制备粒径大小可控、单分散的羧基化PVN 纳米微球，是比 PS 更优的 LETIA 新载体，具有很好的临床应用前景。

Preparation and application of carboxylic polyvinyl naphthalene nanospheres

Using vinyl naphthalene(VN) as the monomer, polyvinyl naphthalene(PVN) nanometer microspheres were synthesized by emulsion polymerization,which were made for immunodetection reagents by carboxylating and coating with β2-microglobulin(β2-M) antibody. β2-M was determined respectively using polystyrene(PS)and PVN immunodetection reagents and the results were statistically processed and analyzed,the purpose of which was that the performance of PVN-homemade nanoparticles carrier for latex enhanced turbidimetric immunoassay (LETIA) was evaluated. Immunological reagents of carboxylated PVN nanospheres had been successfully used for testing standard samples and having good linearity,therefore they were more sensitive than that of PS nanospheres. Control-lable particle size, monodisperse carboxylated PVN nanoparticles can be successfully synthesized by using method and conditions of this paper and have good prospects for clinical application,which are better than that of PS nano-spheres carrier for LETIA.