二硫化碳吸入染毒对雄性大鼠生殖功能及子代影响的研究

目的探讨二硫化碳对雄性大鼠生殖功能及子代的影响。方法选用健康雄性Wistar大鼠40只,随机分为4组,以不同浓度二硫化碳(0、50、250、1250mgm3)静式吸入染毒,共10周。染毒结束前1周随机选对照、低、高浓度组各5只按经典致畸试验与健康雌鼠1∶2合笼交配,观察雌鼠受孕率、计流产数、吸收胎、活胎总数、每窝平均活胎数及称胎鼠体重,检查胎鼠外观及内脏、骨骼畸形,测量身长、尾长、腹围、肛殖距等;染毒结束后,称染毒大鼠体重及各脏器重、计算系数,测量睾丸横径,计附睾尾精子总数、精子活动率及分级、精子畸形率等。结果实验组雌鼠受孕率均低于对照组,但差异无显著性(P>0.05);高浓度组胎鼠各生长发育指标明显低于对照组(P<0.05或P<0.01);染毒大鼠体重、各脏器系数均低于对照组,但只有高浓度组大脑脏器系数与对照组比较差异有显著性(P<0.05);实验组睾丸脏器系数有随染毒剂量增加而降低的趋势,但只有高浓度组与对照组比较有差异(P<0.05);附睾重随染毒剂量增加而减轻,中、高浓度组与对照组比较差异分别有显著性(P<0.05)和极显著性(P<0.01),附睾尾精子总数及精子活动率染毒组均低于对照组,中、高浓度组与对照组比较差异分别有显著性(P<0.05)和极显著性(P<0.01),活动精子分级以原地运动为主。结论二硫化碳染毒对雄性大鼠生殖功能及子代有一定影响,可导致雌鼠受孕率降低、流产率升高,高浓度组胎鼠生长发育迟缓和畸形率增高,可能与雄性大鼠精子质、量下降有关。

Study on the reproductive effects of carbon disulfide in male rats and their subgeneration

OBJECTIVE: To study reproductive toxicity of carbon disulfide and the effects of their subgeneration. METHODS: 40 male rats were randomly divided into four groups. It took ten weeks for the rats to breath CS2 in different densities (0, 50, 250, 1250 mg/m3). Five rats were randomly chosen from the controlling group, high and low dosage group, put them together with female rats for copulation in the ninth week. We observed the pregnant rates, miscarry counts, resorption counts, the numbers and body weight of their subgeneration, weight of the placenta, length of the body, length of the tail, length of the belly, the distance from the rectum to the genital, the effects of the sketetion and the purtenunce. In the eleventh weeks, we measured testide coefficient and testide horizontal of male rats, epididymal weight, sperm count, sperm motility and its classification, the ratio of sperm deformity, etc. RESULTS: The pregnant rats of the dosage groups were all lower than the control group, but the differences were not statistically significant. The data of the high dosage group was obviously lower than the control group (P < 0.05 or P < 0.01). The body weight and the organ coefficient were all lower than the control group, but only brain coefficient of the high group between the control group were statistically significant (P < 0.05). Teside coefficient of the high dosage group obvioulsly decreased than the control group (P < 0.05). Epididymal weight, sperm count, sperm molitity and incidence rate of ospermia of middle and high dosage group obviously decreased than control group. CONCLUSION: The effects of CS2 in distortion rate and abnormal data of growth are possibly related to the decrease in sperm quality and quantity.