组织因子在结肠癌表达的临床意义

目的 探讨组织因子（Tissue factor，TF）在结肠癌表达的临床意义。方法 用RT—PCR和免疫组织化学方法分别检测TF在12例结肠癌旁组织、结肠癌组织中mRNA和蛋白抗原的表达。HPIAS图像分析各组标本相对密度，t检验各组相对密度差异。结果 TF mRNA在癌旁组织中低表达，在结肠癌组织中伴有淋巴结转移和肝转移者TF mRNA表达明显高于无转移者，P〈0．05。TF蛋白抗原在癌旁组织呈阴性和弱阳性。在伴有琳巴结和肝转移组阳性表达率为83．33%高于无转移组66．67％。有差别。但无统计学意义。TF表达与肿瘤的转移力高度相关。结论 TF是反映结肠癌进展和生物学特性的指标，是临床上判断预后指标之一。

Clinical study on expression of tissue factor in human colon carcinoma

[Objective] To study the significance of the expression of tissue factor in colon carcinoma. [Methods] The expression of Tissue factor mRNA and protein antigen were detected by RT-PCR and immunohistology chemical in 12 case colon carcinoma. Expression density of TF in each groups was analyzed with HPIAS image analyzer, compared with t test, the correlation between the expression level of TF and malignancy was tested with Kruskal-Wallis. [Results] The TF mRNA in the tissue around the carcinoma was negative or very low, in the tissue of colon carcinoma, the level of the TF mRNA was higher markedly at the group with lymph node and liver metastasis comparied with no metastasis ,the the value of P is below 0.05. The expression of TF antigen was negative and weak positive in the tissue around the carcinoma, the positive rate was 83.33% in the groups with metastasis, while 66.67% in the groups without metastasis, the difference was exit, but show no Statistical significance. The TF was highly related to the carcinoma's ability of metastasis. [Conclusion] TF is an index which reflecting the colon carcinoma' s process and biology character, It is indicated that TF is a good prognostic factor in clinical diagnosis for colon carcinoma.