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| p53基因治疗与放疗联合作用对卵巢癌细胞的杀伤效果 | |
| 引用本文： | 关婷,金镇,崔满华,邱晓红,李守柔.p53基因治疗与放疗联合作用对卵巢癌细胞的杀伤效果[J].郑州大学学报(医学版),2003,38(6):910-912. |
| 作者姓名： | 关婷 金镇 崔满华 邱晓红 李守柔 |
| 作者单位： | 1. 广州军区广州总医院妇产科,广州,510010 2. 中国医科大学第二临床学院妇产科,沈阳,130003 3. 吉林大学第二临床学院妇产科,长春,130041 |
| 基金项目： | 吉林省卫生科技基金资助项目 981617 |
| 摘 要： | 目的：探讨外源性p53基因转染与放疗联合作用对人卵巢癌细胞的杀伤效果。方法：用脂质体介导的转染技术，将人野生型p53基因的真核表达载体及不含p53基因的空载体分别导入不表达p53的卵巢癌SKOV-3细胞中，经G418筛选，Northern blot及Western blot鉴定后，观察X射线照射对其集落形成的影响。结果：外源性p53基因在转染细胞中有效表达。经X射线照射后，转染p53基因组的SKOV-3细胞的集落形成数与转染空载体组及未转染的SKOV-3细胞组相比，明显降低。结论：外源性p53基因能增加卵巢癌细胞对放射线的敏感性，2者联合作用能更有效地杀灭肿瘤细胞。 |
| 关 键 词： | p53 基因治疗 放疗 卵巢癌 |
| 修稿时间： | 2002年6月3日 |
Efficacy of p53 gene therapy combined with radiotherapy against human ovarian cancer cells |
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| GUAN Ting ,JIN Zhen ,CUI Manhua ,QIU Xiaohong ,LI Shourou.Efficacy of p53 gene therapy combined with radiotherapy against human ovarian cancer cells[J].Journal of Zhengzhou University: Med Sci,2003,38(6):910-912. | |
| Authors: | GUAN Ting JIN Zhen CUI Manhua QIU Xiaohong LI Shourou |
| Institution: | GUAN Ting 1),JIN Zhen 2),CUI Manhua 3),QIU Xiaohong 3),LI Shourou 3) 1)Department of Obstetrics and Gynecology,Guangzhou General Hospital of Guangzhou Military Command,Guangzhou 510010 2)Department of Obstetrics and Gynecology,the Second Clinical College,China Medical University,Shenyang 130003 3)Department of Obstetrics and Gynecology,the Second Clinical College,Jilin University of Medical Sciences,Changchun 130041 |
| Abstract: | Aim:To explore the efficacy of p53 gene therapy combined with radiotherapy against human ovarian cancer cells. Methods:Human wild-type p53 gene eukaryotic expression vector and the same vector without p53 gene were introduced into SKOV-3 cells respectively by lipofectin-mediated gene transfection. The expression of p53 gene was detected by Northern blot and Western blot. The colony formation of transfected cells was observed after irradiating with X-rays.Results:The exogenous p53 gene was effectively expressed in the transfected cells. After irradiating with X-rays, the colony number of cells transfected with p53 gene was much lower than that of transfected and untransfected control. Conclusion:Exogenous wild-type p53 gene can enhance the sensitivity of human ovarian cancer cells to radiotherapy. The combination of p53 gene therapy and radiotherapy may be more effective in ovarian cancer treatment. |
| Keywords: | p53 gene therapy radiotherapy ovarian cancer |
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