不同浓度氨水对小鼠光气染毒的现场防护研究

背景与目的： 检测小鼠光气染毒后肺部氧化指标的改变及不同浓度氨水对其指标的影响，以探讨对光气肺损伤现场防护的新方法。 材料与方法： 动物按体重随机分为5组，每组10只，雌雄各半，分别为阴性对照组，阳性对照组，低剂量(10 ml/L)氨水组，中剂量(20 ml/L)氨水组及高剂量(40 ml/L)氨水组。染毒柜内光气浓度23.8 mg/m3，染毒5 min。其中氨水组染毒柜内同时分别放置10 ml/L、20 ml/L和40 ml/L氨水。染毒后3 h，将动物断头处死，采血并取肺脏。荧光法分别检测肺脏的丙二醛(Malondialdehyde,MDA)、还原性谷胱甘肽(Reduced glutathione,GSH)和氧化性谷胱甘肽(Oxidized glutathione,GSSG)以及全血谷胱甘肽过氧化物酶(Glu tathione peroxidase,GSH_Px)，同时进行全血分析。 结果： 阴性对照组与阳性对照组各项指标比较差异均有统计学意义；且不同剂量氨水组肺脏的MDA、GSSG含量均有减少，而肺脏GSH、全血GSH_Px差异无统计学意义。此外，各组WBC与阳性对照组比较有显著下降，RBC、HGB、PLT各组相似。 结论： 光气中毒后肺部有氧化损伤，暴露于光气时给予挥发性氨水进行现场消毒，可以减轻这种氧化损伤。

The Protective Effects of Ammonia Against Phosgene Lung Toxicity

BACKROUND&AIM: To discuss the protective effects of ammonia against phosgene on mice,in order to find a new therapy for lung injury induced by phosgene. MATERIAL AND METHODS: During phosgene exposure,the treated groups were protected by different dose of ammonia.After3h,all mice were anatomized to measure malondialdehyde(MDA),reduced glutathione(GSH),oxidized glutathione(GSSG)of lung and glutathione peroxidase(GSH-Px)of blood by fluorescence-metry. RESULTS: MDA,GSH,GSSG were different significantly between control and positive group.With compared with positive group,MDA and GSSG of trial group was lower(P<0.05). CONCLUSION: There were some oxidative injury occurred in lung during phosgene exposure and ammonia may have protective effect on it.