大鼠中央杏仁核-卵圆核投射神经元的免疫细胞化学特征——荧光金逆行追踪结合免疫荧光双标记法研究

本实验用雄性Sprague-Dawley大鼠,全身麻醉下将荧光金水溶液注入一侧终纹床核群卵圆核区域,在同侧中央杏仁核看到大量逆行标记细胞。将此切片分别用促肾上腺皮质激素释放因子、神经降压素、甲-脑啡肽和胆囊收缩素抗血清孵育,再用FITC结合的羊抗兔IgG温育,结果在中央杏仁核范围内,看到数量不等的荧光金逆行标记细胞,分别被上述4种神经肽免疫血清标记。本组实验结果表明,中央杏仁核有上述4种肽能神经元投射到终纹床核群的卵圆核区域。

IMMUNOCYTOCHEMICAL PROPERTIES OF NEURONS IN THE CENTRAL NUCLEUS OF THE AMYGDALA PROJECTING TO THE OVAL NUCLEUS OF THE BED NUCLEI OF THE STRIA TERMINALIS IN THE RAT: A COMBINED FLUOROGOLD RETROGRADE TRACT-TRACING AND IMMUNOFLUORESCENCE STAINING STU

The central nucleus of the amygdala(Ce) is heterogeneous in cyto- and chemoarchitecture, and contains over a dozen neuropeptides. Our recent works suggested that there were reciprocal connections between the Ce and the oval nucleus (Or) of the bed nuclei of the stria terminalis (BST). The present experiment was designed to study the immunocytochemical properties of the Ce neurons projecting to the Ov by the fluoro-gold(FG) retrograde tract-tracing method combined with indirect immunofluorescence staining in male Sprague-Dawley rats.It was found that the neurons in the Ce were labeled densely following injection of FG into the Ov, and some FG labeled neurons were restained by indirect immunofluorescence procedures using antisera to corticotropin-releasing factor (CRF), neurotensin (NT), M-enkephalin(M-ENK) or cholecystokinin(CCK). The findings suggest that the CRF-, NT-, M-ENK- and CCK-containig neurons in the Ce project to the Ov.