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| 透明质酸的瘤学分布与p-CLP示踪学研究 | |
| 引用本文： | 刘志强,强永刚,梁智欣,廖永华. 透明质酸的瘤学分布与p-CLP示踪学研究[J]. 中华肿瘤防治杂志, 2010, 17(3) |
| 作者姓名： | 刘志强 强永刚 梁智欣 廖永华 |
| 作者单位： | 广州医学院实验核医学教研室,广东,广州,510182 |
| 基金项目： | 国家自然科学基金(30670622); 广州科技计划项目(2008J1-C261); 广州市教育局项目(61049) |
| 摘 要： | 目的:确定透明质酸(HA)瘤内分布,为肺肿瘤的早期诊断提供依据。方法:免疫组化定位肿瘤内HA的分布,亲和层析法从仔猪肋软骨提取猪软骨链蛋白(p-CLP),125I标记p-CLP进行体内分布代谢示踪学研究。结果:HA免疫显色提示肿瘤细胞外基质HA有较高表达,放射示踪实验示1～7h内实验组单位质量的放射性计数百分比(13.46%～29.14%)明显高于对照组(6.4%～13.70%),差异有统计学意义,P<0.01。结论:p-CLP在肺肿瘤内浓集,125I标记p-CLP体内代谢示踪能够为肺肿瘤等疾病的早期诊断提供实验依据。 |
| 关 键 词： | 透明质酸 组比分布 肺肿瘤 放射性示踪剂 |
Distribution of hyaluronic acid in lung tumor and piglet cartilage link protein's tracer kinetics |
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| LIU Zhi-qiang,QIANG Yong-gang,LIANG Zhi-xin,LIAO Yong-hua. Distribution of hyaluronic acid in lung tumor and piglet cartilage link protein's tracer kinetics[J]. Chinese Journal of Cancer Prevention and Treatment, 2010, 17(3) | |
| Authors: | LIU Zhi-qiang QIANG Yong-gang LIANG Zhi-xin LIAO Yong-hua |
| Affiliation: | LIU+Zhi-qiang,QIANG+Yong-gang,LIANG+Zhi-xin,LIAO+Yong-hua+Department+of+Experimental+Nuclear+Medicine,Guangzhou+Medical+College,Guangzhou+510182,P.R.China |
| Abstract: | OBJECTIVE:To determine the distribution of hyaluronic acid (HA) in lung tumor so as to provide an experimental evidence for early diagnosis of lung tumor.METHODS:The distribution of HA in tumor was determined by immunohistochmistry location,piglet cartilage link protein (p-CLP) was purified by affinity chromatography from piglet costal,and the tracer studies of p-CLP,which were used to detect the tissue distribution of HA indirectly,were determined in vivo by 125I-p-CLP,which was labeled with Na125I.RESULTS... |
| Keywords: | hyaluronic acid tissue distribution lung neoplasms radioactive tracers |
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