Expression of the large plastid gene,ORF2280, in tomato fruits and flowers |
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Authors: | Christina M. Richards Ross C. Hardison Charles D. Boyer |
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Affiliation: | (1) Department of Horticulture, The Pennsylvania State University, 16802 University Park, PA, USA;(2) Department of Biochemistry and Molecular Biology, The Pennsylvania State University, 16802 University Park, PA, USA;(3) Present address: Department of Horticulture, Oregon State University, 97331 Corvallis, OR, USA |
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Abstract: | ![]() The expression of ORF2280, a large plastid gene of unknown function, was examined in tomato leaves, in a developmental series of tomato fruits, and in tomato flowers. Western blots indicated that much more ORF2280 protein is present in fruits and flowers than in leaves. The most abundant proteins detected, 68 and 59 kDa, are present in about equal amounts in fruits of all stages; they are even more abundant in flowers. A 170-kDa ORF2280 protein is also present in fruit of all stages; it is most abundant in small green fruit. The presence of higher levels of ORF2280 proteins in tomato fruits and flowers indicates that it may have a specialized function in these nonphotosynthetic tissues. |
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Keywords: | Unidentified open reading frame Plastid gene expression Lycopersicon esculentum Fruit ripening |
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