The molecular mass of adenovirus type 5 as determined by means of scanning transmission electron microscopy (STEM) |
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| Authors: | R W Ruigrok M V Nermut P J Andree |
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| Affiliation: | 1. Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands;2. National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, U.K. |
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| Abstract: | The mass of adenovirus type 5 was determined by means of computer-assisted scanning transmission electron microscopy (STEM). Arithmetic mean of 157 +/- 10(SD) X 10(6) daltons and mode between 160 and 170 X 10(6) daltons compare favourably with previously reported data. The advantages of the STEM-procedure over the physical and chemical techniques are: low amounts of purified virus particles are needed; visual control of the physical state of virus particles; no need to know the chemical composition or protein concentration of the virus sample. |
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| Keywords: | adenovirus type 5 scanning transmission electron microscopy (STEM) mass determination |
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