透镜诱导型近视眼视网膜色素上皮细胞肝细胞生长因子表达的变化

目的观察豚鼠镜片诱导型近视眼（LIM）眼球前部及后极部视网膜色素上皮（RPE）细胞中肝细胞生长因子（HGF）的表达变化，探讨近视眼的发病机制。方法取2周龄豚鼠10只，随机选择一眼作为实验眼，制备近视眼模型（眼前配戴凹透镜45d），对侧眼作为自身对照。实验前后测量豚鼠双眼屈光度和眼轴长度。原代培养豚鼠视网膜前部及后极部RPE细胞，传1代后，对各组RPE细胞HGF表达进行免疫细胞化学分析。采用SPSS12．0统计软件对相关数据进行配对t检验和单因素方差分析。结果①豚鼠经过45d的透镜诱导后，形成（-6．70±1．93）D的相对近视，眼轴延长（1．53±0．31）mm，前后差异有统计学意义（t=-9．25，t=9．17，P〈0．05），近视模型造模成功。②在光镜下观察免疫细胞化学爬片显示．HGF存RPE细胞中的表达定位于细胞浆，细胞核无着色。近视眼前部和后极部RPE细胞的HGF表达明最高于自身对照组，差异有统计学意义（P〈0．05）；近视模型眼和对照眼自身前部与后极部HGF表达比较，差异无统计学意义（P〉0．05）。结论体外培养的豚鼠RPE细胞稳定表达HGF：HGF在近视模型眼前部和后极部RPE细胞中的活性都升高，参与了实验性近视眼的形成。

Expression of hepatocyte growth factor in guinea pig retinal pigment epithelial cells

Objective To observe the expression of hepatocyte growth factor (HGF) in anterior and posterior retinal pigment epithelial (RPE) cells of guinea pig lens-induced myopia;to discuss the pathological mechanism of experimental myopia. Methods Ten two-week-old guinea pigs were ran-domly fitted with monocular lenses. Before and after the experiment, refractive error and the length of the optic axis were measured. The RPE cells of each group were cultured and passaged one genera-tion in vitro, Immunocytochemistry was used for RPE cell identification and to detect the expression of hepatocyte growth factor (HGF) in RPE cells. These data were statistically analyzed by SPSS 12.0 expressed in cytoplasm and was detected in all anterior and posterior RPE cells. The expression of HGF in experimental eyes was higher than in control eyes (P<0.05). Expression of HGF was almost the same between anterior and posterior sections (P>0.05). There was a significant difference in the expression of HGF between experimental eyes and control eyes (P<0.05). Conclusion Expression of HGF is detected in guinea pig RPE cells. It shows that HGF plays a role in the development of myopia.