青藤碱对吗啡依赖小鼠、大鼠及豚鼠离体回肠催促戒断反应的影响

目的 :研究青藤碱 (sinomenine ,SIN)对吗啡依赖小鼠、大鼠及豚鼠离体回肠催促戒断反应的抑制作用。方法 :连续递增sc吗啡 (morphine ,Mor) ,建立大鼠、小鼠及豚鼠吗啡身体依赖性模型 ;纳洛酮 (naloxone,Nal)催促诱发吗啡身体依赖性豚鼠离体回肠的戒断性收缩。结果 :(1)SIN 14.3 - 143mg·kg- 1 显著抑制纳洛酮催促后 3 0min内小鼠的跳台次数 ;(2 )SIN 10 0mg·kg- 1 降低纳洛酮催促后 1h内大鼠的戒断症状分值及抑制体重下降 ;(3 )SIN(1.5×10 - 4 - 6.0× 10 - 4 mol·L- 1 )剂量依赖性地抑制纳洛酮催促诱发的吗啡身体依赖性豚鼠离体回肠的戒断性收缩 ;(4)吗啡身体依赖性豚鼠ipSIN(87.0和 8.7mg·kg- 1 )后 ,其离体回肠经纳洛酮催促诱发的戒断性收缩幅值显著降低。结论 :SIN对吗啡身体依赖性大鼠、小鼠的戒断症状及豚鼠离体回肠的戒断性收缩具有抑制作用

EFFECTS OF SINOMENINE ON MORPHINE WITHDRAWAL SYNDROMES IN MICE、RATS AND GUINEA PIG ILEUM

Objective : To study the effect of sinomenine(SIN) on morphine withdrawal syndromes in mice, rats and ileum from guinea pigs. Methods : Morphine-dependent animal models, including mice, rats and guinea pigs, were set up by continuous subcutaneous administration of morphine. The withdrawal contractures in ileum from morphine-dependent guinea pigs were precipitated by naloxone. Results : (1) SIN( 14 3- 143 mg·kg -1 ) reduced the jumping incidence and jumping times within half an hour after precipitated by naloxone in morphine-dependent mice;(2) SIN(100 mg·kg -1 ) inhibited the naloxone-precipitated withdrawal syndromes and body weight loss in morphine-dependent rats;(3)SIN(1.5×10 -4 -6.0×10 -4 mol·L -1 ) dose-dependently inhibited naloxone-precipitated contractures in ileum from morphine-dependent guinea pigs.;(4) the intraperitoneal administration of SIN(87.0 and 8.7 mg·kg -1 ) reduced naloxone-precipitated contractures in ileum from morphine-dependent guinea pigs. Conclusion : SIN could inhibit morphine withdrawal syndromes in mice or rats and the withdrawal contractures of in vitro ileum from morphine-dependent guinea pigs.