响应面分析法优化藤茶中二氢杨梅素的提取工艺

目的:优选藤茶中二氢杨梅素的提取工艺.方法:以二氢杨梅素提取率为考察指标,在单因素试验基础上,选取乙醇体积分数、料液比、提取时间为自变量,二氢杨梅素提取率为响应值,采用三因素三水平的响应面分析法优选二氢杨梅素的提取工艺条件.结果:最佳提取工艺条件为17倍量81％乙醇回流提取95 min.在此条件下,二氢杨梅素提取率可达9.58％,与理论值9.72％仅相差0.14％.结论:采用响应面法优选的二氢杨梅素提取工艺是可行的.

Optimization of Extraction Technology for Dihydromyricetin from Ampelopsis grossedentata by Response Surface Analysis Methodology

Objective: To optimize extraction process of dihydromyricetin from Ampelopsis grossedentata by response surface methodology. Method: With extraction ratio of dihydromyricetin as index,based on single factor test,the concentration of ethanol, liquid-solid ratio and extraction time were selected as independent variables and extraction rate of dihydromyricetin as response value,response surface methodology with three-factors and three-levels was carried out for optimizing extraction process of dihydromyricetin from A. grossedentata. Result: Optimum extraction conditions of dihydromyricetin were as followed:refluxing extracted 95 min with 17 times the amount of 81% ethanol. Under these conditions,extraction ratio of dihydromyricetin was 9.58%,relative error in comparison with the predicted one was 0.14%. Conclusion: This optimized technology was reliable,it showed that was feasible for optimizing extraction technology of dihydromyricetin by response surface methodology.