藏药镰形棘豆中鼠李柠檬素对照品的制备

目的:建立镰形棘豆中鼠李柠檬素对照品的制备方法.方法:运用硅胶柱色谱、Sephndex LH-20凝胶色谱柱等分离纯化的方法制备鼠李柠檬素,采用薄层色谱法和高效液相色谱面积归一化法检测纯度,波谱法鉴定其结构.结果:制备的化合物为鼠李柠檬素,纯度＞98％.结论:该制备方法效率高,所得鼠李柠檬素对照品纯度高,符合中药化学对照品的相关技术要求,可用于大量制备中药定性、定量分析使用的对照品.

Preparation of Rhamnocitrin Reference Substances from Oxytropis Falcata

Objective: To establish a method for the preparation of rhamnocitrin reference substances from Oxytropis falcata. Method: Separated using silica gel column chromatography preliminarily, and then separated and purified rhamnocitrin by Sephdex LH-20 column, thin-layer chromatography and area normalization of HPLC was used to detect purity, spectral analysis identified its structure. Result: The preparation of compounds of rhamnocitrin, had a purity of more than 98%. Conclusion: The preparation method has high efficiency, the reference substance induced has high purity and meet the relevant technical requirements of traditional Chinese medicine chemical reference substance, can be used for mass production of the reference substance used for traditional Chinese medicine qualitative and quantitative analysis.