罗汉果皂苷抗疲劳及耐缺氧作用

目的:考察罗汉果皂苷的抗疲劳、耐缺氧作用.方法:将小鼠随机分为空白组,模型组,阳性对照组(红景天胶囊570 mg· kg-1),罗汉果皂苷高剂量( 300 mg· kg-1),中剂量(150 mg·kg-1)及低剂量组(75 mg·kg-1).连续灌胃给药21 d后采用力竭游泳实验和常压耐缺氧实验,考察罗汉果皂苷对小鼠的抗疲劳和耐缺氧能力的影响,并测定服用罗汉果皂苷后肝糖原和肌糖原的变化,及运动后血尿素氮、血乳酸含量和乳酸脱氢酶活力的变化.结果:罗汉果皂苷显著延长小鼠力竭游泳时间(P＜0.05),150,75 mg·kg-1组小鼠耐缺氧存活时间显著延长(P＜0.05).服用罗汉果皂苷后,小鼠肝糖原和肌糖原含量均显著增加(P ＜0.001),运动后血尿素氮和乳酸生成显著减少(P ＜0.001),乳酸脱氢酶活性显著增强(P＜0.05).结论:罗汉果皂苷能显著增强小鼠的抗疲劳和耐缺氧能力,其作用可能与增加机体糖原储备和加速乳酸代谢有关.

Anti-fatigue and Hypoxia Tolerance Effect of Mogrosides in Mice

Objective: To evaluate anti-fatigue and hypoxia tolerance effect of mogrosides in mice. Method: Mice were randomly divvied into normal control group, model control group, positive control group (Hongjingtian capsule, 570 mg·kg^-1), mogrosides high dosage (300 mg·kg^-1), medium dosage (150 mg·kg^-1) and low dosage groups (75 mg·kg^-1). After orally administrated for 21 days consecutively, exhaustive swimming test and atmospheric hypoxia test were employed to evaluate anti-fatigue and hypoxia tolerance effect of mogrosides in mice. Furthermore, liver glycogen and muscle glycogen level were examined, urea nitrogen level, lactic acid level and lactate dehydrogenase activity after swim were determined. Result: Mogrosides significantly extended swimming time (P<0.05), and extended hypoxia survival time at 75 mg·kg^-1 and 150 mg·kg^-1 (P<0.05). Moreover, liver glycogen and muscle glycogen level were significantly increased after treatment (P<0.001), urea nitrogen level, lactic acid level were decreased (P<0.001), lactate dehydrogenase activity was enhanced (P<0.05). Conclusion: Mogrosides possesses abilities of anti-fatigue and hypoxia tolerance. The effects could be mediated by increased glycogen supplying and improved lactate metabolism.