| 急性腹泻患儿诺如病毒检测和分型 |
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| 引用本文： | 薛华,翟菊萍.急性腹泻患儿诺如病毒检测和分型[J].中国现代医生,2011,49(31):84-85. |
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| 作者姓名： | 薛华 翟菊萍 |
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| 作者单位： | 1. 苏州市金闾区疾病预防保健中心,江苏苏州,215008
2. 苏州大学附属第一医院检验科,江苏苏州,215006 |
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| 摘 要： | 目的了解苏州地区急性腹泻患儿诺如病毒的分子特征和基因型别。方法收集苏州地区2009年9月-2010年3月共153份5岁以下急性腹泻患儿粪便或肛拭标本，用多重RT—PCR方法检测诺如病毒，选择部分阳性扩增片段进行基因序列测定，并对序列进行系统进化分析。结果急性腹泻患儿诺如病毒检出率为16．34％（25／153），均为诺如病毒Ⅱ型，且诺如病毒基因型别均为GⅡ-4／2006b变异株。结论诺如病毒是2009～2010年引起苏州地区急性腹泻患儿重要的病原之一，诺如病毒GⅡ-4／2006b变异株或类似株可能是2009～2010年苏州地区流行的优势株。
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| 关 键 词： | 诺如病毒 分子特征 基因型别 |
Detection and Typing of Norovirus of Acute Diarrhea in Suzhou Area, 2009-2010 |
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| Authors: | XUE Hua ZHAI Juping |
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| Institution: | XUE Hua, ZHAI duping (1.Jinchang District Center for Disease Prevention and Control, Suzhou 215008, China; 2.Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou 215006, China) |
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| Abstract: | Objective To investigate the molecular characteristics and genotypes of human Norovirus （NV） in acute diarrhea in Suzhou. Methods Fecal specimens were collected from children （ 〈 5 years） with acute diarrhea. Norovirus of 1.53 specimens were detected by multiplex RT-PCR. PCR products of several positive samples were randomly selected and sequenced. The sequences were analyzed by phylogenetic analysis. Results Twenty-five Norovirus positive cases were detected in 153 facal specimens （16.34%）, 25 isolates were Norovirus II -types. Conclusion Norovirus is one of the major pathogens causing acute diarrhea of children, and G II - 4/2006b variant and like strains are probably identified as the prevalent strain from 2009 to 2010 in Suzhou, Jiangsu Province. |
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| Keywords: | Norovirus Molecular characteristics Genotype |
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