| 黄连解毒汤对体外白念珠菌生物膜形成的影响 |
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| 引用本文： | 汪长中,程惠娟,官妍,汪本凡,王艳,汤华阳,乐红霞,宋功道.黄连解毒汤对体外白念珠菌生物膜形成的影响[J].热带病与寄生虫学,2007,5(2):82-84. |
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| 作者姓名： | 汪长中 程惠娟 官妍 汪本凡 王艳 汤华阳 乐红霞 宋功道 |
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| 作者单位： | 安徽中医学院中西医结合临床学院,合肥,230000 |
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| 摘 要： | 目的观察黄连解毒汤体外对白念珠菌生物膜形成的影响。方法采用结晶紫染色法和MTT法评价白念珠菌生物膜的体外生长动力学,微量稀释法检测黄连解毒汤对白念珠菌悬浮菌及其生物膜的抑制作用以及药物包被对白念珠菌生物膜形成作用。结果生物膜内白念珠菌数量随培养时间延长而增加。黄连解毒汤对白念珠菌悬浮菌的最低抑菌浓度50%(MIC50)为3.125mg/ml,对生物膜的SMIC50与SMIC80分别是3.125和6.25mg/ml。药物包被浓度在1.56mg/ml以上时对其生物膜形成的抑制作用有显著性。结论黄连解毒汤对体外白念珠菌生物膜具有明显的抑制作用。
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| 关 键 词： | 黄连解毒汤 白念珠菌 生物膜 |
| 修稿时间： | 2007年4月14日 |
Effect of Huanglianjiedu decoction on Candida albicans biofilm formation in vitro |
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| Wang Changzhong,Cheng Huijuan,Guan Yan,Wang Benfan,Wang yan,Tang Huayang,Le Hongxia,Song Gongdao.Effect of Huanglianjiedu decoction on Candida albicans biofilm formation in vitro[J].Journal of Tropical Diseases and Parasitology,2007,5(2):82-84. |
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| Authors: | Wang Changzhong Cheng Huijuan Guan Yan Wang Benfan Wang yan Tang Huayang Le Hongxia Song Gongdao |
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| Abstract: | Objective To observe the effect of Huanglianjiedu decoction(HLJDD) on C.albicans biofilm formation in vitro. Methods Crystal violet staining and MTT assay were performed to evaluate the growth kinetics on C.albicans biofilm formation in vitro,Trace dilution was used to detected the susceptibility of planktonic and biofilm C.albicans to HLJDD,and the influence of coating the wells of a microtiter plate with HLJDD on C.albicans biofilm. Results The biomass of C.albicans increased with culturing time. The planktonic MIC50 and sessile MIC(SMIC50 ,SMIC80) values of C.albicans to HLJD were 3.125 mg/ml,3.125 mg/ml and 6.25 mg/ml,respectively. The coating of a microtiter plate with HLJDD had an inhibitory effect on subsequent biofilm formation. Conclusion HLJDD could significantly inhibit C.albicans biofilm formation in vitro. |
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| Keywords: | Huanglianjiedu decoction (HLJDD) C albicans Biofilm |
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