| N-甲基-D-天冬氨酸对神经细胞内游离钙水平的影响 |
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| 引用本文： | 冯杰 李奎刘欣,许梓荣. N-甲基-D-天冬氨酸对神经细胞内游离钙水平的影响[J]. 营养学报, 2005, 27(5): 425-427 |
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| 作者姓名： | 冯杰 李奎刘欣 许梓荣 |
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| 作者单位： | 浙江大学动物分子营养学教育部重点实验室 杭州310029(冯杰,李奎,刘欣),浙江大学动物分子营养学教育部重点实验室 杭州310029(许梓荣) |
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| 基金项目： | 国家自然科学基金(No.30000120) |
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| 摘 要： | 目的:探讨N-甲基-D-天冬氨酸(NMDA)对神经细胞内游离钙离子浓度的影响,并初步探讨其作用机制。方法:采用钙离子荧光指示剂Fura-2/AM和双波长检测技术测定细胞内游离钙的浓度。结果:1×10-8～1×10-4mol/LNMDA可使大鼠神经细胞内游离钙[Ca2+]i水平显著升高(P<0.05),且呈剂量和时间依赖关系。结论:NMDA升高神经细胞内[Ca2+]i的作用与胞外Ca2+大量内流和胞内Ca2+储池释放有关,且以胞外Ca2+内流为主。胞外Ca2+内流不仅与NMDA受体门控Ca2+通道和电压门控Ca2+通道有关,还与电压依赖性Na+通道有关。
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| 关 键 词： | N-甲基-D-天冬氨酸(NMDA) 细胞内游离钙 神经细胞 荧光指示剂 |
| 文章编号： | 0512-7955(2005)-05-0425-03 |
| 收稿时间： | 2005-02-28 |
| 修稿时间： | 2005-02-28 |
EFFECTS OF N-METHYL-D-ASPARTATE ON INTRACELLULAR FREE CALCIUM ION CONCENTRATION IN NEURONS |
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| FENG Jie,LI Kui,LIU Xin,XU Zi-rong. EFFECTS OF N-METHYL-D-ASPARTATE ON INTRACELLULAR FREE CALCIUM ION CONCENTRATION IN NEURONS[J]. Acta Nutrimenta Sinica, 2005, 27(5): 425-427 |
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| Authors: | FENG Jie LI Kui LIU Xin XU Zi-rong |
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| Abstract: | Objective: To investigate the effects of N-methyl-D-aspartate(NMDA) on intracellular free calcium[Ca2 ]i dissociated neurons in newborn rats and its possible mechanism. Methods: Calcium fluorescent indicator fura-2/AM was used. Results: NMDA could increase the level of intracellular free Ca2 of corticocerebral neurons significantly(P<0.05), and the concentrations of [Ca2 ]i response to NMDA were dose-time dependent. Conclusion:NMDA-induced increases in [Ca2 ]i were associated with the influx of Ca2 from outside of cells and release of stored Ca2 , mainly with the influx of Ca2 from outside of cells. Furthermore, the influx Ca2 from outside of the cells was related not only to NMDA receptor gated Ca2 channel and voltage-gated Ca2 channel, but also to the voltage-dependent Na channel. |
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| Keywords: | N-methyl-D-aspartate(NMDA) intracellular free calcium neurons Fura-2/AM |
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