EpCAM和CD44在胃癌中的表达及临床意义

通过检测上皮细胞粘附分子（epithelial cell adhesion molecule,Ep CAM）、CD44在不同胃组织中的表达差异,探讨其与胃癌发生、浸润和转移的关系。方法采用免疫组化法检测经病理证实的胃腺癌组织标本42例、不典型增生组织标本25例和正常胃组织标本25例中Ep CAM和CD44的表达,分析其表达与临床病理参数间的关系,及Ep CAM和CD44表达的相关性。结果 Ep CAM在胃腺癌组织、不典型增生组织和正常胃组织中的阳性表达率两两比较差异均有统计学意义（88.1%,64.0%,8.0%,P均〈0.01）,在胃腺癌组织中表达与淋巴结转移、浆膜侵润和TNM分期（Ⅰ~Ⅱ/Ⅲ~Ⅳ）有关（P均〈0.05）;CD44在胃腺癌组织中、不典型增生组织和正常胃组织的阳性表达率两两比较亦均有统计学差异（69.4%,40.0%,12.0%,P均〈0.01）,其在胃腺癌组织中表达与胃癌TNM分期、分化程度（中高分化/低分化）和淋巴结转移有关（P〈0.05,P〈0.01）。Ep CAM和CD44在胃腺癌组织中的表达呈正相关（r=0.363,P〈0.01）。结论 Ep CAM和CD44在胃癌的发生、发展中起重要作用,与胃癌的浸润、转移有关,联合检测可作为胃癌早期诊断、判断预后的的指标。

Expressions of EpCAM and CD44 in gastric carcinoma and their clinical significance

To study the difference of epithelial cell adhesion molecule （EpCAM） and CD44 expressions in different gastric tissues for exploring its association with oncogenesis, invasion and metastasis of gastric carcinoma. Methods The specimens of 42 gastric adenocarcinoma tissues, 25 atypical hyperplasia tissues and 25 normal gastric tissues were collected. The EpCAM and CD44 expressions in the tissues were detected by immunohistochemistry SP method, and the relationships between their expression and clinical pathological parameters and the correlation of EpCA and CD44 expressions were analyzed. Results By pairwise comparison, there were all significant differences in the positive rates of EpCAM in gastric carcinoma tissues, atypical hyperplasia tissue and normal tissue （88.1% ,64.0% , 8.0% ,all P 〈 0.01 ）, and its expression in gastric adenocarcinoma tissue was related to lymph node metastasis, serosal invasion and TNM stage （ Ⅰ -Ⅱ/Ⅲ-Ⅳ ） （all P 〈 0.05 ）. By pairwise comparison, there were also significant differences in the positive rates of CD44 in gastric carcinoma tissues, atypical hyperplasia tissues and normal tissues （ 69.4%, 40. 0% ,12.0% ,all P 〈0.01 ） , and its expression in gastric adenocarcinoma tissues was related to TNM stage （ Ⅰ -Ⅱ/Ⅲ- Ⅳ ,P 〈0. 01 ）, differentiation degree （ high-mid/low, P 〈 0.05 ） and lymph node metastasis （ P 〈 0.01 ）. There was positive correlation between the expressions of EpCAM and CD44 in gastric adenocarcinoma tissues （ r =0. 363 ,P 〈0. 01 ）. Conclusions EpCAM and CD44 play an important role in the occurrence and development of gastric carcinoma and are involved in its invasion and metastasis. Their combined detection can be used as the index of early diagnosis and judgment of prognostic of gastric cancer.