Identification and localization of SAS-6 in the microsporidium Nosema bombycis |
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| Institution: | 1. Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, China;2. Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, Jiangsu Province, China;1. Kunming National High-level Biosafety Research Center, Chinese Academy of Medical Science, Institute of Medical Biology, China;2. Kunming City Center for Disease Control and Prevention, China;1. Division of Clinical Research, The First Hospital of Jilin University, Changchun, Jilin 130021, China;2. Department of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100020, China;3. Division of Education, Beijing Jishuitan Hospital, Beijing 100020, China;4. Department of Hepatology, The First Hospital of Jilin University, Changchun, Jilin 130021, China;5. Maternal and Child Health Center of Chaoyang District, Beijing 100020, China;1. Vavilov Institute of General Genetics, Moscow 119991, Russia;2. Sechenov University, Moscow 119435, Russia;3. Moscow Region State University, Moscow 105005, Russia;4. Sanitary-Epidemiological Department of Kyrgyz Republic MOH, Bishkek, Kyrgyzstan;1. Guangdong Provincial Center for Disease Control and Prevention, 160 Qunxian Road, Panyu District, Guangzhou 511430, Guangdong, China;2. Guangdong Provincial Institute of Public Health, Guangdong Provincial Center for Disease Control and Prevention, 160 Qunxian Road, Panyu District, Guangzhou 511430, Guangdong, China;1. KU Leuven, Department of Microbiology and Immunology, Rega Institute, Laboratory of Evolutionary and Computational Virology, 3000, Leuven, Belgium;2. Baqiyatallah Research Center for Gastroenterology and Liver Disease, Baqiyatallah University of Medical Sciences, Tehran, Iran;3. Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, 4032 Debrecen, Hungary;4. Biosafety Development Group, Cellular Sciences Department, Amgen Inc., One Amgen Center Drive, Thousand Oaks, CA 91320, USA;5. KU Leuven, Department of Microbiology and Immunology, Rega Institute, Laboratory of Clinical and Epidemiological Virology, 3000, Leuven, Belgium;6. Blood Transfusion Research Centre, High Institute for Research and Education in Transfusion Medicine, Hemmat Exp. Way, 14665-1157, Tehran, Iran |
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| Abstract: | The centriole in eukaryotes functions as the cell's microtubule-organizing center (MTOC) to nucleate spindle assembly. The evolutionarily conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis. Microsporidia possess the spindle plaque instead of centriole as their MTOC to nucleate spindle assembly. However, little is known about the components of spindle plaques in microsporidia. In our present study, we identified a SAS-6 protein in the microsporidium Nosema bombycis and named it as NSAS-6. The NSAS-6 gene contains a complete ORF of 1104 bp in length that encodes a 367-amino acid polypeptide. NSAS-6 consists of a conserved N-terminal domain and a coiled-coil domain. The high identity of SAS-6 homologous sequences from microsporidia indicates that SAS-6 is a conserved protein in microsporidia. Immunolocalization in sporoplasms, intracellular stages and mature spores showed that NSAS-6 probably localizes to the nucleus of N. bombycis and exists throughout the life cycle of N. bombycis. These results suggest that NSAS-6 is required in cell morphogenesis and division in N. bombycis. The function and structure of NSAS-6 should be the focus for further studies, which is essential to elucidate the role of SAS-6 in spindle plaque assembly. |
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