中药复方君子汤对白血病细胞株K562增殖和凋亡的影响

本研究探讨中药复方君子汤（FFJZ）对抑制白血病K562细胞生长增殖和诱导凋亡的生物学特点及其可能的作用机制。应用细胞形态学、台盼蓝拒染法观察细胞生长状态;四氮甲唑蓝（MTT）法检测细胞增殖情况;流式细胞术检测细胞的凋亡率。结果表明：一定浓度范围的FFJZ作用于K562细胞后,随FFJZ药物浓度的升高其对K562细胞生长的抑制率明显增加（p〈0.01）,其半数抑制浓度（IC50）约为5.6mg/ml。在FFJZ浓度为4mg/ml时,细胞凋亡以早期凋亡为主,而至8mg/ml组时晚期凋亡比例显著增多,与未加药组比较有显著差异（p〈0.01）。结论：在体外FFJZ一定浓度范围内可以抑制白血病K562细胞的生长增殖,诱导其凋亡并呈浓度依赖性,其机制可能与诱导细胞凋亡有关。

Effects of Chinese Medicine Compound FFJZ on Proliferation and Apoptosis of Leukemia Cell Line K562

This study was purposed to explore the biological effects suppressing growth and inducing apoptosis of Chinese medicine compound FFJZ on leukemia cell line K562 and its possible mechanisms of FFJZ. The growth status of K562 cells cultured in vitro was determined by trypan blue exclusion test; the suppressive effect of FFJZ on K562 cells was assayed by MTT method; the inducing apoptosis of FFJZ on K562 cells was detected by flow cytometry. The results showed that after K562 cells were treated with FFJZ in certain concentration range,the inhibited rate of FFJZ on K562 cell growth was remarkably increased along with enhancement of FFJZ,the IC50 value of FFJZ on K562 cells was 5.6 mg/ml after treatment for 48 hours. At 4 mg/ml of FFJZ the early apoptosis predominated in K562 cells,at 8 mg/ml of FFJZ the late apoptosis ratio significantly increased. As compared with control group without FFJZ,there was significant difference （p0.01）. It is concluded that the FFJZ in range of certain concentration can suppress growth and proliferation of K562 cells and induce their apoptosis in concentration-dependent manner,the mechanism of which may be associated to inducing apoptosis of K562 cells.