| 海芒果种子提取物nerifolin对人肝癌细胞系HepG2增殖和凋亡的影响 |
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| 引用本文： | 陈若华,蒲瑾,戴焱焱,黄才国,楼国良.海芒果种子提取物nerifolin对人肝癌细胞系HepG2增殖和凋亡的影响[J].中国肿瘤生物治疗杂志,2011,18(1):51-54. |
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| 作者姓名： | 陈若华 蒲瑾 戴焱焱 黄才国 楼国良 |
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| 作者单位： | 1. 第二军医大学,长海医院,特需诊疗科,上海,200433
2. 第二军医大学基础部生化与分子生物学教研室,上海,200433 |
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| 基金项目： | 国家高科技研究发展计划（863计划）项目资助（No. 2006AAD9Z447） |
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| 摘 要： | 目的:观察来源于海芒果种子的皂苷类化合物nerifolin对人肝癌细胞HepG2增殖及凋亡的影响,初步探讨其作用机制。方法:MTT法检测不同质量浓度nerifolin对HepG2细胞增殖的影响,流式细胞术检测nerifolin对HepG2细胞周期和凋亡的影响。Caspase试剂盒检测nerifolin对HepG2细胞caspase-3酶活化的影响。结果:Nerifolin可时间和剂量依赖性地抑制HepG2细胞增殖,作用24、48、72h的IC50分别为(2.34±0.08)、(0.13±0.01)、(0.06±0.01)μg/ml。随着nerifolin(0.1μg/ml)作用时间的延长,HepG2细胞S期百分比逐渐增多,而G0/G1期百分比逐渐减少(P<0.01),nerifolin阻滞HepG2细胞于S期。Nerifolin作用后,HepG2细胞早期凋亡率上升至22.65%,caspase-3活性比对照组明显升高(P<0.01)。结论:Neri-folin可通过S期阻滞抑制HepG2细胞的增殖,通过caspase-3依赖途径诱导HepG2细胞的凋亡。
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| 关 键 词： | 肝癌HepG2 nerifolin 增殖 凋亡 细胞周期 caspase-3 |
| 收稿时间： | 2010/10/20 0:00:00 |
| 修稿时间： | 2010/12/22 0:00:00 |
Effects of nerifolin,an isolate from seeds of Cerbera manghas L.,on proliferation and apoptosis of human hepatocellular carcinoma HepG2 cells |
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| CHEN Ruo-hu,PU Jin,DAI Yan-yan,HUANG Cai-guo and LOU Guo-liang.Effects of nerifolin,an isolate from seeds of Cerbera manghas L.,on proliferation and apoptosis of human hepatocellular carcinoma HepG2 cells[J].Chinese Journal of Cancer Biotherapy,2011,18(1):51-54. |
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| Authors: | CHEN Ruo-hu PU Jin DAI Yan-yan HUANG Cai-guo and LOU Guo-liang |
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| Institution: | VIP Clinical Department, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;VIP Clinical Department, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;VIP Clinical Department, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China;VIP Clinical Department, Changhai Hospital, Second Military Medical University, Shanghai 200433, China |
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| Abstract: | |
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| Keywords: | liver cancer HepG2 nerifolin proliferation apoptosis cell cycle caspase-3 |
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