喉癌中细胞外基质金属蛋白酶诱导因子和基质金属蛋白酶2、9的表达及其临床意义

目的 探讨喉癌中细胞外基质金属蛋白酶诱导因子(CD147)和基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)的蛋白表达与肿瘤浸润、转移和预后的关系.方法 应用免疫组化EnVision二步法检测48例喉癌、15例喉炎组织中CD147、MMP-2、MMP-9的表达,并与临床病理特征和预后进行对比.结果 CD147、MMP-2、MMP-9在喉癌的阳性表达率分别为87.5%、75.0%、79.2%,明显高于喉炎组26.7%、6.7%、33.3%(P＜0.01);CD147、MMP-2、MMP-9的阳性表达均与喉癌临床分期和淋巴结转移有关;3项指标在喉癌中的表达具有相关性(P＜0.01);单因素生存分析显示CD147和MMP-2、MMP-9在表达阴性的5年生存率(分别为83.3%、83.3%、90.0%)高于表达阳性(25.0%、7.7%、18.2%),差异均有统计学意义(均P＜0.05);多元回归分析证实MMP-9是危险因子,其表达越高患者预后越差(P＜O.05).结论 CD147、MMP-2、MMP-9与喉癌侵袭、转移有关;CD147与MMP-2、MMP-9的产生有密切的关系;MMP-9可能成为独立的预后判断因子.

Expressions of CD147, MMP-2 and MMP-9 in laryngeal carcinoma and clinical significance

Objective To investigate the expressions of extracellular matrix metalloproteinase inducer ( CD147 ), matrix metalloproteinases-2 ( MMP-2 ) and matrix metalloproteinases-9 ( MMP-9 ) in human laryngeal carcinoma and study their relationship with invasion, metastasis and prognosis. Methods The EnVision immunohistochemical method was used to detect the expressions of CD147, MMP-2 and MMP-9 in 48 cases of laryngeal carcinoma and 15 cases of laryngitis. The results were compared with their clinical pathological features and prognosis. Results The positive expression rates of CD147, MMP-2 and MMP-9 were significantly higher in laryngeal carcinoma than those in laryngitis ( 87. 5%, 75.0% , 79. 2% vs 26. 7%, 6. 7%, 33.3% ); there was a positive correlation between a high-level expression of CD147,MMP-2, MMP-9 and clinical stage and the status of lymph node metastasis( P ＜ 0. 01 ) ;univatiate analysis indicated that the overall survival rate was higher in patients with a negative expression of CD147, MMP-2,MMP-9 than those with a positive expression ( 83.3%, 83.3%, 90. 0% vs 25.0%, 7.7%, 18. 2% );multivariate analysis showed that MMP-9 was a risk predictor. A higher expression of MMP-9 was associated with a shorter survival time. Conclusion CD147 and MMP-2 play a role in invasion and metastasis of laryngeal carcinoma; increased levels of MMP-2 and MMP-9 are induced by CD147 in laryngeal tumor cells;MMP-9 may be an independent prognostic factor.