| 怀山药的离体繁殖 |
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| 引用本文： | 李明军,李金亭,朱命炜,张嘉宝.怀山药的离体繁殖[J].中草药,1999,30(4):296-298. |
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| 作者姓名： | 李明军 李金亭 朱命炜 张嘉宝 |
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| 作者单位： | 河南师范大学生物系!新乡453002 |
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| 基金项目： | 河南省自然科学基金，省科技攻关项目 |
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| 摘 要： | 怀山药带节茎段的ＭＳ＋６－ＢＡ１－２ｍｇ／Ｌ＋ＮＡＡ０．１－１ｍｇ／Ｌ的培养基上培养，均能直接形成多芽体，均芽数为３－５。将多芽体转和ＭＳ或ＭＳ＋ＫＴ２ｍｇ／Ｌ＋ＮＡＡ０．０２ｍｇ／Ｌ＋ＰＰ３３３０．１ｍｇ／Ｌ的培养基上均能诱导生根，形成再生植株。但后者形成的苗生长健壮。叶色浓绿，有较强的生活力。
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| 关 键 词： | 怀山药 节培养 多芽体 再生植株 离体繁殖 |
| 收稿时间： | 1998/7/20 0:00:00 |
n vitro Propagation of Common Yam (Dioscorea opposita) |
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| Li Mingjun,Li Jinting,Zhu Mingwei.n vitro Propagation of Common Yam (Dioscorea opposita)[J].Chinese Traditional and Herbal Drugs,1999,30(4):296-298. |
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| Authors: | Li Mingjun Li Jinting Zhu Mingwei |
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| Institution: | Department of Biology,Henan Normal University,Xinxiang 453002;Department of Biology,Henan Normal University,Xinxiang 453002;Department of Biology,Henan Normal University,Xinxiang 453002;Department of Biology,Henan Normal University,Xinxiang 453002 |
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| Abstract: | Dioscorea opposita Thunb. was micropropagated from nodal culture. This species produced 3 ~5 buds per node when cultured on modified MS Medium supplemented with 6-BA 1-2mg/L and NAA 0.1 --1 mg/L. Root could be induced when these multibuds were transplanted onto MS basic Medium or MS basic Medium supplemented with KT 2 mg/L, PP333 0.1 mg/L and NAA 0.02 mg/L. The regenerated plantlets treated with PP333, grew robustly and developed deep green leaves with vigour. |
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| Keywords: | Dioscorea opposita Thunb nodal culture multiple buds regenerated plantlets |
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