| 阻断MEK／ERK上调内质网应激条件下CHOP和p53表达 |
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| 引用本文： | 代荣阳,严冬梅,段春燕,李洪. 阻断MEK／ERK上调内质网应激条件下CHOP和p53表达[J]. 泸州医学院学报, 2009, 32(5): 461-464 |
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| 作者姓名： | 代荣阳 严冬梅 段春燕 李洪 |
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| 作者单位： | 泸州医学院生物化学教研室,四川泸州,646000 |
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| 摘 要： | 目的：研究MEK／ERK信号途径在肝癌细胞SMMC-7721抵抗内质网应激诱导凋亡中的分子机制。方法：采用MEK特异抑制剂PD98059阻断内质网应激介导的MEK／ERK活化，并利用流式细胞和Western blot技术分析阻断MEK／ERK对内质网应激诱导的细胞凋亡及其关键调控分子GRP78、CHOP和p53表达的影响。结果：阻断MEK／ERK信号途径后，SMMC-7721细胞对内质网应激诱导凋亡的敏感性明显增强，CHOP和p53的蛋白水平显著上调。结论：内质网应激介导的MEK／ERK活化能够通过下调CHOP和p53表达抵抗细胞凋亡。
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| 关 键 词： | MEK/ERK信号途径 内质网应激 细胞凋亡 SMMC-7721 |
INHIBITION OF MEK UP-REGULATES THE EXPRESSION OF CHOP AND P53 UNDER ER STRESS |
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| Dai Rongyang,et al. INHIBITION OF MEK UP-REGULATES THE EXPRESSION OF CHOP AND P53 UNDER ER STRESS[J]. Journal of Luzhou Medical College, 2009, 32(5): 461-464 |
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| Authors: | Dai Rongyang et al |
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| Affiliation: | Dai Rongyang,et al Department of Biochemistry,Luzhou Medical College |
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| Abstract: | Objective:To investigate the molecular mechanism of MEK/ERK pathway in inhibiting ER stressinduced apoptosis of SMMC-7721 cells.Methods:MEK/ERK was blocked by MEK specific inhibitor PD98059,and its effects on ER stress-induced SMMC-7721 cells apoptosis and the expression of GRP78,CHOP and p53 wrer analyzed by flow cytometry and Western blot analysis.Results:SMMC-7721 cells were relatively resistant to ER stress-induced apoptosis.After MEK/ERK was blocked,SMMC-7721 cells were significantly sensitized to dith... |
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| Keywords: | SMMC-7721 |
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