反相高效液相色谱法同时测定脉络宁注射液中6种酚酸类成分的含量

目的 建立用反相高效液相色谱法同时测定脉络宁注射液中6种酚酸含量的方法。方法采用Kromasil-C18柱（4．6mm×250mm，5μm）；流动相：乙腈-0．1％磷酸溶液，梯度洗脱；流速：1．0mL／min；紫外检测波长：327nm；柱温：25℃；进样量：20gL。结果新绿原酸、绿原酸、隐绿原酸、咖啡酸、3，5-二咖啡酰奎宁酸、3，4-二咖啡酰奎宁酸的线性范围分别为0．01090～0．5448μg（r=0．9999）、0．02406～1．2032μg（r=1）、0．01106～0．5528μg（r=1）、0．01594～0．7968μg（r=1）、0．009104～0．4552μg（r=1）、0．01037～0．5184μg（r=1），回收率分别为101．06％、100．22％、101．54％、99．42％、100．21％、101．65％。结论 本法灵敏简便，准确可靠，可作为脉络宁注射液中新绿原酸、绿原酸、隐绿原酸、咖啡酸、3，5-二酰奎宁酸、3，4-二咖啡酰奎宁酸含量同时测定的方法。

Determination of Six Phendic Acids in Mailuoning Injection by RP-HPLC

Objective To establish a method for determination the contents of six phendic acids in Mailuoning injection by HPLC at same time. Methods Separation was performed on a Kromasil-C18 （4.6 mm× 250 mm, 5 μm） column with mobile phase consisting of 0.1% phosphoric acid-water and Acetonitrile with gradient elute at the flow rate of 1 mL/min. The UV detection wavelength was set at 327 nm and the column temperature was set at 25℃. The sample size was 20 μL. Results The standard cures of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, 3,5-Dicaffeoylquinic acid, 3,4- Dicaffeoylquinic acid were linear within the contentration range of 0.010 90-0.544 8μg （r =0.999 9）, 0.02406-1.2032 μg （r= 1）, 0.01106-0.552 8 μg （r= 1）, 0.01594-0.7968 μg （r= 1）, 0.009 104-0.4552 μg （r = 1）, 0.010 37-0.518 4 μg （r = 1） respectively. And the recovery rates of them were 101.06%, 100.22%, 101.54%, 99.42%, 100.21%, 101.65% respectively. Conclusion The method appeared to be simple, reliable, accurate and can be used to determine the contents of six phendic acids in Mailuoning injection.