实验性精索静脉曲张大鼠睾丸氧化应激水平的检测

目的测定青春期精索静脉曲张（VC）大鼠睾丸组织中氧化应激水平,探讨VC致不育的机制。方法将40只雄性青春期Wistar大鼠随机分为VC 8周组、VC 12周组（各12只）和相应的假手术对照组（各8只）,通过部分结扎左肾静脉建立实验性大鼠VC模型,对照组只游离左肾静脉不结扎。分别于术后8、12周解剖动物,测左侧精索静脉最大直径;比色法测睾丸组织内过氧化氢（H2O2）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和总抗氧化能力（T-AOC）水平。结果成功建立了VC模型。VC 8周组和12周组大鼠左侧精索静脉均明显扩张（t=7.19、6.86,P〈0.01）,双侧睾丸组织内H2O2含量较对照组明显增加（t=2.31~4.78,P〈0.05、0.01）,与右侧相比左侧增加更明显（t=2.56、3.15,P〈0.05）,并且左侧VC 12周组比VC 8周组增加更明显（t=2.16,P〈0.05）。VC 8周组和VC 12周组左侧CAT活力与对照组比较显著降低（t=2.23、3.03,P〈0.05、0.01）,右侧数值有下降趋势但差异无显著性（t=0.95、1.41,P〉0.05）。VC 8周组和VC 12周组双侧SOD活力和T-AOC与对照组比较均明显降低（t=3.29~4.72,P〈0.01）,并且VC 12周组比VC 8周组降低更明显（t=2.80、3.38,P〈0.05、0.01）,T-AOC左侧睾丸比右侧下降更明显（t=11.66、7.54,P〈0.01）。结论 VC可引起双侧睾丸H2O2增加,CAT活力、SOD活力以及T-AOC下降,导致睾丸组织内氧化应激系统失衡。

DETECTION OF TESTICULAR OXIDATIVE STRESS LEVEL IN EXPERIMENTAL VARICOCELE IN RATS

Objective To detect the level of oxidative stress in testis of experimental adolescent varicocele in rats and study its mechanism of infertility. Methods A model of varicocele was created in 24 adolescent male Wistar rats by partially ligating the left renal veins,which were equally randomized to group A and group B（varicocele for 8 and 12 weeks,respectively）.Eight rats underwent sham-operation and another eight served as a control group received only a dissection of the left renal vein.The animals were dissected at 8 and 12 weeks after surgery and the maximum diameter of the left spermatic veins measured,levels of H2O2,CAT,SOD and T-AOC of the testes checked biochemically. Results A model of varicocele was successfully created.Compared with the corresponding control groups,the diameter of the left spermatic veins were significantly enlarged（t=7.19,6.86;P0.01）;the levels of H2O2 in both testes of groups A and B significantly increased（t=2.31,P0.05;t=3.59,4.78,P0.01）,that of the left side increased more（t=2.56,3.15;P0.05） and that in group B was much higher than that in group A（t=2.16,P0.05）.Activity of CAT of left testes in groups A and B more reduced than that of the controls（t=2.23,P0.05;t=3.03,P0.01）,while that of the right testis had a downturn but with no statistical significance（t=0.95,1.41;P0.05）.Levels of SOD and T-AOC of the both-side in both varicocele groups were lower than that of the control（t=3.29-4.72,P0.01）,and more remarkable reduction was seen in group B than in group A（t=2.80,P0.05;t=3.38,P0.01）,the T-AOC of the left testis was lower than that of the right（t=11.66,7.54;P0.01）. Conclusion Varicocele induces the increase of H2O2 content of both testes,decrease of vigor of CAT,SOD and T-AOC,resulting in disbalance of oxidative stress response system in testis.