蝌蚪提取液诱导人早幼粒白血病细胞分化的实验研究

目的 :探讨蝌蝌提取液 (T871)对白血病细胞的诱导分化作用。方法 :利用细胞生长曲线、形态学观察、细胞免疫化学及原位mRNA杂交和完整细胞原位斑点印迹技术 ,观察了T871诱导HL 60细胞分化的作用和分化过程中癌基因c myc、c myb表达的变化。结果 :T871能抑制HL 60细胞的增殖 ,形态学表现为类似单核 /巨噬细胞 ,同时T871还能使HL 60细胞的NBT还原能力、ANAE活性显著增强 ,同时伴有癌基因c myc、c mybmRNA表达的下调。结论 :T871能诱导HL 60细胞向单核 /巨噬细胞方向分化 ,c myc、c myb癌基因可能参与了此过程

Induction of differentiation on human promyelocytic leukemia cells by tadpole extract

Objective: To investigate the induction of differentiation by tadpole extract (T871) on leukemia cells. Methods: We studied the effects of T871 on proliferation and differentiation of HL 60 cells by the cell growth stage , cytomorphological observation , cytochemical techniques. We also examined the onco gene (c myc, c myb) expresion during the induction of differentiation in T871 treated HL 60 cells by in situ hybridization and intact cell mRNA dot blot techniques. Result: T871 was able to inhibit the proliferatin of HL 60 cells. T871 might also induce HL 60 cells to monocyte macrophage like cells as shown by cytomorphological observation , enhancing of NBT reducing ability, increasing of ANAE,activity . the differentiation of HL 60 cells was accompanied by downregulation of c myc , c myb gene expression. Conclusion: Tadpole extract may induce HL 60 cells to differentiate along the monocyte macrophage pathway , onco gene c myc , c myb may take part in the course.