双黄连的提取精制工艺研究

采用亚沸态水提，絮凝和醇沉相结合的方法实现双黄连的提取精制，达到提高中药有效成分的目的。得到最佳方案为：经超临界萃取后的100g药材，加入10倍体积的水，在90℃下煎煮2h，过滤，滤液浓缩至80mL，在50℃下，加入1．5％的絮凝剂133mL，搅拌5min，离心，上清液浓缩至16mL，加入95％乙醇至含醇量为80％，过滤，滤液蒸干，得率约为5-6％。

Improved Techniques for Extracting Shuanghuanglian

This is an improved approach to cut down the needed dosage, extraction, 1.5% chitosan flocculation, and alcohol precipitation using a combined process of sub-boiling water to extract and purify medicinal materials in Shuanghuanglian. It goes through the following procedure： extract medicinal materials from Shuanghuanglian using supercritical-CO2 fluid, before boiling them in water 10 times of the extracts at a temperature of 90℃ for 2 hours. The filtrate is concentrated to 80ml, and combined with 133ml of 1.5% Chitosan flocculating agent at a temperature of 50℃. Stir and centrifuge the compound for 5 minutes, and obtain a concentrated supernate of 16mL. Mix the compound with 95%-alcohol, making the content of ethanol up to 80%. A further filtration and drying process allows an extraction rate up to 5-6 %.