同种异体脂肪干细胞复合脱钙骨材料修复兔尺骨缺损

目的：探讨同种异体脂肪干细胞（adipose—derivedstemcells，ADSCs）组织工程骨修复兔管状骨缺损的可行性。方法：获取新西兰大白兔的肩胛部脂肪，分离培养具有多向分化潜能的ADSCs；第三代兔ADSCs与脱钙骨复合后，体外成骨诱导培养（LG—DMEM）设为对照。制造兔两侧尺骨临界大小（长度15mm）的缺损，分别植入兔ADSCs一脱钙骨复合物（实验侧）和单纯脱钙骨材料（对照侧）；12周后取样本，三维CT和组织学检测观察成骨情况。结果：细胞一材料复合物植入12周后，三维CT显示实验侧有新生骨基质长成，对照侧未见骨组织生成；组织学检测显示实验侧缺损区被典型的骨组织取代，可见新生骨小梁附着于脱钙骨表面，而对照侧只有少量的骨组织和纤维组织充填。结论：兔ADSCs能在脱钙骨上很好的黏附和生长，兔ADSCs-脱钙骨材料复合物植人体内能成功修复临界大小的管状骨缺损。

The effect of allogeneic rabbit adipose-derived stem cells combined with decalcified bone material in the repair of ulna defect in rabbit

Objective：To investigate the feasibility of allogeneic adipose-derived stem cells（ADSCs） in tissue engineering bone repair of tubular bone defects. Methods ：The rabbit ADSCs were isolated and cultured from scapular fat of New Zealand white rabbits. Passage 3 rADSCs were seeded onto demineralized bone matrix （DBM） and osteoinduetively cultured, LG-DMEM was set as control group. Bilateral critical-sized bone defects（ 15 ram） of ulna were created. One side of the defect was repaired with rADSCs-DBM（ experimental group）, another side of the defect was repaired with only DBM（ control group）. The osteogenesis was observed by three-dimensional CT and histological examination at 12 weeks after treatment. Results： At 12 weeks after implantation, three-dimensional CT scan showed that new bones formed in experimental group, while no bone tissue formation in control group. Histological examination revealed that the defect was replaced by typical bone tissue in experimental side, while only minimal bone formation and fibrous connection was observed in control group. Conclusions ： rADSCs can well adhere and grow in the surface of DBM. Rabbit ADSCs combined with decalcified bone material can successfully repair the critical-sized tubular bone defects.