| Identification and Characterization of Peptide Mimics of Blood Group A Antigen |
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| 作者姓名： | 汤兆明 王琳 胡丽华 李一荣 崔天盆 熊娟 窦丽芳 |
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| 作者单位： | Department of Laboratory Medicine Union Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430022，China，Department of Laboratory Medicine，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430022，China，Department of Laboratory Medicine，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430022，China，Department of Laboratory Medicine，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430022，China，Department of Laboratory Medicine，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430022，China，Department of Laboratory Medicine，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430022，China，Department of Laboratory Medicine，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430022，China |
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| 基金项目： | Acknowledgement We thank Dr. Yuan TIAN for his technical assistance. |
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| 摘 要： | In order to investigate peptide mimics of carbohydrate blood group A antigen, a phage display 12-mer peptide library was screened with a monoclonal antibody against blood group A antigen, NaM87-1F6. The antibody-binding properties of the selected phage peptides were evaluated by phage ELISA and phage capture assay. The peptides were co-expressed as glutathione S-transferase （GST） fusion proteins. RBC agglutination inhibition assay was performed to assess the natural blood group A antigen-mimicking ability of the fusion proteins. The results showed that seven phage clones selected bound to NaM87-1F6 specifically, among which, 6 clones bore the same peptide sequence, EYWYCGMNRTGC and another harbored a different one QIWYERTLPFTF. The two peptides were successfully expressed at the N terminal of GST protein. Both of the fusion proteins inhibited the RBC agglutination mediated by anti-A serum in a concentration-dependent manner. These results suggested that the fusion proteins based on the selected peptides could mimic the blood group A antigen and might be used as anti-A antibody-adsorbing materials when immunoabsorption was applied in ABO incompatible transplantation.
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| 关 键 词： | 鉴别方法 缩氨酸 抗原 血型 |
Identification and characterization of peptide mimics of blood group A antigen |
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| Zhaoming?Tang,Lin?Wang,Lihua?Hu,Yirong?Li,Tianpen?Cui,Juan?Xiong,Lifang?Dou.Identification and Characterization of Peptide Mimics of Blood Group A Antigen[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2008,28(2):222-226. |
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| Authors: | Zhaoming Tang Lin Wang Lihua Hu Yirong Li Tianpen Cui Juan Xiong Lifang Dou |
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| Institution: | Department of Laboratory Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
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| Abstract: | In order to investigate peptide mimics of carbohydrate blood group A antigen,a phage display 12-mer peptide library was screened with a monoclonal antibody against blood group A anti-gen,NaM87-1F6.The antibody-binding properties of the selected phage peptides were evaluated by phage ELISA and phage capture assay.The peptides were co-expressed as glutathione S-transferase(GST) fusion proteins.RBC agglutination inhibition assay was performed to assess the natural blood group A antigen-mimicking ability of the fusion proteins.The results showed that seven phage clones selected bound to NaM87-1F6 specifically,among which,6 clones bore the same peptide sequence,EYWYCGMNRTGC and another harbored a different one QIWYERTLPFTF.The two peptides were successfully expressed at the N terminal of GST protein.Both of the fusion proteins inhibited the RBC agglutination mediated by anti-A serum in a concentration-dependent manner.These results suggested that the fusion proteins based on the selected peptides could mimic the blood group A an-tigen and might be used as anti-A antibody-adsorbing materials when immunoabsorption was applied in ABO incompatible transplantation. |
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| Keywords: | amino acid sequence blood group A antigen hemagglutination test molecular mimicry peptide library |
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