三种方法检测人乳头瘤病毒的比较

目的比较基因芯片分型法、PCR荧光定量法、免疫组织化学法检测人乳头瘤病毒（humanpa pillomavirus，HPV）的敏感性，为临床人乳头瘤病毒检测提供适用的方法。方法收集2002至2007年在本院就诊的宫颈癌病例200例，采用新鲜宫颈癌组织进行人乳头瘤病毒基因芯片分型检测；在上述200例患者中进取自愿接受荧光定量PCR检测的患者134例，收取宫颈癌灶分泌物，采用荧光定量PCR检测方法检测人乳头瘤病毒；对在上述134例宫颈癌患者中，选取自愿接受免疫组织化学检测的患者利用石蜡包埋宫颈癌组织，采用免疫组织化学法检测人乳头瘤病毒。结果基因芯片分型法检测人乳头瘤病毒呈阳性为188例，阳性率为94．0％（188／200），其中HPV-16／-18阳性率为95．74％（180／188）。荧光定量PCR法检测HPV-16／18阳性率为36．57％（49／134）；免疫组化法检测HPV-16／18阳性率为46．51％（60／129）。荧光定量PCR检测和免疫组化法检测人乳头瘤病毒呈阳性者，在基因芯片分型法检测中均呈阳性。基因芯片分型法人乳头瘤病毒检出率与荧光定量PCR检测和免疫组化法比较，差异有显著意义（P〈O．05），而后两种方法比较，差异无显著意义（P〉0．05）。结论基因芯片分型技术检测人乳头瘤病毒具有敏感性好，且同时可以分型的明显优势，适用于临床人乳头瘤病毒感染的诊断和研究。

Comparison of Three Methods to Detect the Human Papilloma Virus

Objective To compare the susceptibility of human papilloma virus （HPV） by the detecting methods of genotyping chip system, fluorescence quantitative PCR and immunohistochemistry. Methods From 2002 to 2007, a total of 200 fresh freezen tissues of cervical cancer were detected for the susceptibility of human papilloma virus by the human papilloma virus genotyping chip system, 134 secretion of cervical lesion were detected by fluorescence quantitative PCR, and 129 paraffin imbedded of cervical cancer were detected by immunohistochemistry. Results The human papilloma virus positive rate of the genotyping chip method was 94.00~ （188/200）, among them, HPV-16/-18 accounted for 95.74~/oo （180/ 188）. The positive rate of HPV-16/-18 which was detected by the fluorescence quantitative PCR was 36. 570//oo （49/134）. The positive rate of HPV-16/-18 which was detected by immunohistochemical method was 46. 51% （60/129）. The positive cases who were detected by fluorescence quantitative PCR and immunohistochemistry were all positive in human papilloma virus genotyping chip system. There had statistical difference of detecting rates among human papilloma virus genotyping chip method and fluorescence quantitative PCR or immunohistochemistry （P~0.05）, while significance difference was not found between fluorescence quantitative PCR and immunohistochemistry （P ~ 0. 05）. Conclusion The human papilloma virus genotyping chip system is good and can divide the types, meanwhile its outcomes are objective and directly perceived through the senses. Human papilloma virus genotyping chip system is suitable to clinical diagnosis and study.