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| HPLC法检测人血浆甲砜霉素浓度 | |
| 引用本文： | 丁细桃,孙未,邱相君,胡国新. HPLC法检测人血浆甲砜霉素浓度[J]. 中国药师, 2011, 14(2): 194-196 |
| 作者姓名： | 丁细桃 孙未 邱相君 胡国新 |
| 作者单位： | 1. 温州医学院机能实验教学中心,浙江温州,325035 2. 温州医学院药学院药理学教研室 3. 河南科技大学医学院药理学教研室 |
| 摘 要： | 目的:建立人血浆甲砜霉素检测的高效液相色谱方法。方法:血浆经乙酸乙酯-正己烷萃取,色谱柱:Agilent TC-C18;流动相:乙腈-0.1%三氟乙酸-水（20:20:60）,流速:0.8 ml·min-1;检测波长:225 nm。结果:甲砜霉素浓度在0.10～10.00 mg·L-1范围内线性关系良好（r=0.999 9）;高中低三个浓度(0.25,2.50,7.50 mg·L-1)的相对回收率分别为（99.20±4.41）%、（98.07±3.94）%和（100.07±1.69）%;日内RSD分别为5.28%、5.19%和1.97%,日间RSD分别为4.38%、3.95%和1.33%。结论:本方法简便、快速、准确可靠,适用于人血浆甲砜霉素浓度的测定及其药代动力学研究。 |
| 关 键 词： | 甲砜霉素 药代动力学 高效液相色谱法 |
Determination of Thiamphenicol in Human Plasma by HPLC |
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| Ding Xitao,Sun Wei,Qiu Xiangjun,Hu Guoxin. Determination of Thiamphenicol in Human Plasma by HPLC[J]. China Pharmacist, 2011, 14(2): 194-196 | |
| Authors: | Ding Xitao Sun Wei Qiu Xiangjun Hu Guoxin |
| Affiliation: | Ding Xitao~1,Sun Wei~2,Qiu Xiangjun~3,Hu Guoxin~2(1.Lab and Teaching Center of Functional Science,Wenzhou Medical College,Zhejiang Wenzhou 325035,2.Department of Pharmacology,3.Department of Pharmacology,Medical College of Henan University of Science and Technology) |
| Abstract: | Objective： To develop an HPLC method for the determination of thiamphenicol in human plasma. Method： The plasma was extracted by ethyl acetate and n-henaxe. The analytical column was packed with Agilent TC-C18. The mobile phase was acetonitrile- 0. 1% trifluoroacetic acid-water （20： 20： 60） and the flow rate was 0. 8 ml .min-1. The UV detection wavelength was 225 nm. Result： Excellent linear relationship was obtained from 0. 10 mg. L-1 to 10. 00 mg. L-1 （ r = 0. 999 9 ）. The relative recovery was （ 99.20 ± 4. 41 ） % , （98. 07 ± 3. 94） % and （ 100. 07± 1.69 ） % at three concentrations, respectively. The intra-day RSD was 5.28%, 5. 19% and 1.97% and inter-day RSD was 4. 38% ,3.95% and 1.33% , respectively. Conclusion： The method is simple, rapid and accurate and can be used to determine the thiarnphenicol concentration in human plasma and study its pharmacokinetics. |
| Keywords: | Thiamphenicol Pharmacokinetics HPLC |
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