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| 荧光光谱法研究青蒿素及双氢青蒿素与肝微粒体的相互作用 | |
| 引用本文： | 孙坤,张爱平,王锐利,李赟,张淑秋. 荧光光谱法研究青蒿素及双氢青蒿素与肝微粒体的相互作用[J]. 中国药物与临床, 2011, 11(6): 617-620 |
| 作者姓名： | 孙坤 张爱平 王锐利 李赟 张淑秋 |
| 作者单位： | 山西医科大学药学院临床药学教研室,太原,030001 |
| 基金项目： | 国家自然科学基金，山西省回国留学人员科研项目，山西医科大学青年基金 |
| 摘 要： | 目的研究抗疟药青蒿素(ART)与双氢青蒿素(DHA)和大鼠肝微粒体(RLM)以及人肝微粒体(HLM)之间的相互作用。方法在模拟人体生理条件下,采用荧光光谱法,得出青蒿素、DHA与RLM和HLM的结合参数。结果青蒿素和DHA对RLM和HLM的猝灭机制不同,它们对RLM的猝灭机制均为静态猝灭,而对HLM的猝灭作用为混合猝灭;其相互间作用力均为氢键和范德华力,其结合反应均为自发的热力学反应。结论青蒿素和DHA对大鼠和人肝微粒体均有结合,且结合力存在种属差异,同时青蒿素和DHA与同种肝微粒体的结合能力也存在差异。 |
| 关 键 词： | 光谱法,荧光 青蒿素 微粒体,肝 |
Spectroscopic study on the interactions between artemisinin or dihydroartemisinin and rat or human liver microsomes |
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| SUN Kun,ZHANG Ai-ping,WANG Rui-li,LI Yun,ZHANG Shu-qiu. Spectroscopic study on the interactions between artemisinin or dihydroartemisinin and rat or human liver microsomes[J]. Chinese Remedies & Clinics, 2011, 11(6): 617-620 | |
| Authors: | SUN Kun ZHANG Ai-ping WANG Rui-li LI Yun ZHANG Shu-qiu |
| Affiliation: | SUN Kun,ZHANG Ai-ping,WANG Rui-li,LI Yun,ZHANG Shu-qiu.Department of Pharmacy,Shanxi Medical University,Taiyuan 030001,China |
| Abstract: | Objective To investigate the interaction between antimalarial drug artemisinin (ART) or dihy droartemisinin (DHA) and rat liver microsomes (RLM) or human liver microsomes (HLM).Methods The Binding parameters of ART or DHA with RLM or HLM were studied under simulated physiological conditions by fluorescience spectra.Results For both reactions of ART and DHA to RLM,the quenching mechanisms were static quenchings,but to HLM,they were mixed static and dynamic quenchings.All of the reactions were spontaneous the... |
| Keywords: | Spectrometry fluorescence Artemisinin Microsomes liver |
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