HPLC法测定酚咖片中有关物质对氨基酚的含量

目的:建立酚咖片中有关物质对氛基酚含量的HPLC测定方法.方法:Diamonsil C18(4.6mm×200mm,5um)色谱柱,0.05mol.L(-1)醋酸按溶液为流动相A,甲醇为流动相B,进行梯度洗脱;检测波长为232 nm,结果:对氨基酚在5-15mg.L(-1)的浓度范围内线性关系良好(r = 0.999 ...

The Quantitative Determination of Related Substance p-Aminophenol in Paracetamol and Caffeine Tablets by HPLC

Objective ：To establish an HPLC method for determination of related substance p-aminophenol in Paracetamol and Caffeine Tablets. Methods：The analytical column was Diamonsil C18 （4. 6 mm × 200 mm, 5 μm） ; The mobile phase A was 0. 05 mol·L^-1 ammonium acetate solution, and the mobile phase B was methanol, using gradient elution ; The detective wavelength was 232 nm. Results：Under this condition, there was a good linear relationship for p-aminophenol in the concentration range of 5 - 15 mg·L^-1 , r = 0. 999 7. The detection limit was 4. 2 × 10^-4 μg （S/N =3）. The average recovery was 100. 1% and RSD =0. 42%. Conclusion：The proposed method enables accurate, sensitive, rapid analysis for p-aminophenol in Paraeetamol and Caffeine Tablets.